Distribution of Gaba in the Nerve Ganglia of Haliotis asinina Linnaeus

N Soonklang; Michael J Stewart; C Wanichanon; Praphaporn Stewart; P J Hanna; P Sabhon

doi:10.2983/035.032.0111

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Distribution of Gaba in the Nerve Ganglia of Haliotis asinina Linnaeus

Journal article

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Distribution of Gaba in the Nerve Ganglia of Haliotis asinina Linnaeus

N Soonklang, Michael J Stewart, C Wanichanon, Praphaporn Stewart, P J Hanna and P Sabhon

Journal of Shellfish Research, Vol.32(1), pp.59-66

2013

DOI: https://doi.org/10.2983/035.032.0111

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Abstract

gamma-aminobutyric acid

Haliotis asinina

ganglia

immunohistochemistry

localization

Gamma-aminobutyric acid (GABA) is a major neurotransmitter and effective settlement inducer in abalone aquaculture. This study aimed to explore the distribution of GABA within neural tissues of Haliotis asinina. Gamma-aminobutyric acid was found in neuronal cell type 1 of 3 major ganglia (i.e., cerebral, pleuropedal, and visceral ganglia) of both sexes. The distribution of GABA-immunoreactive (-ir) cells in the cerebral ganglion was concentrated mostly in the cortex region of the dorsal horn, whereas it was scattered throughout the pleuropedal ganglion, with more in the upper half. Gamma-aminobutyric acid-ir nerve fibers were found throughout the neuropils of the ganglia. The visceral ganglion had the least numbers of GABA-ir neurons compared with the other 2 ganglia. The cells were distributed mainly in the dorsal horn. We also observed GABA to be colocalized with 2 other neurotransmitters: serotonin (5-HT) and dopamine (DA). In the cerebral ganglion, fluorescence double staining of GABA and 5-HT, and GABA and DA showed immunoreactivity in separate cells and was also colocalized in the same cells. In the pleuropedal ganglion, the staining pattern was similar to the cerebral ganglion, but positive-staining cells were less numerous. In the visceral ganglion, GABA and DA, and GABA and 5-HT were colocalized in the same cell types. Overall, we found that GABAergic cells were most numerous in the cerebral ganglion of H. asinina. Further studies are required to determine the functions of these neurotransmitters in relation to their distribution.

Details

Title: Distribution of Gaba in the Nerve Ganglia of Haliotis asinina Linnaeus
Authors: N Soonklang (Author) - Thammasat University, Thailand
Michael J Stewart (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
C Wanichanon (Author) - Mahidol University, Thailand
Praphaporn Stewart (Author) - Thammasat University, Thailand
P J Hanna (Author) - Mahidol University, Thailand
P Sabhon (Author) - Mahidol University, Thailand
Publication details: Journal of Shellfish Research, Vol.32(1), pp.59-66
Publisher: National Shellfisheries Association, Inc.
Date published: 2013
DOI: 10.2983/035.032.0111
ISSN: 0730-8000
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99448788702621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Fisheries; Marine & Freshwater Biology