Journal article
Distribution of Chlamydia pneumoniae DNA in atherosclerotic carotid arteries: Significance for sampling procedures
Journal of Clinical Microbiology, Vol.41(4), pp.1454-1457
2003
Abstract
Despite extensive efforts to confirm a direct association between Chlamydia pneumoniae and atherosclerosis, different laboratories continue to report a large variability in detection rates. In this study, we analyzed multiple sections from atherosclerotic carotid arteries from 10 endartectomy patients to determine the location of C. pneumoniae DNA and the number of sections of the plaque required for analysis to obtain a 95% confidence of detecting the bacterium. A sensitive nested PCR assay detected C. pneumoniae DNA in all patients at one or more locations within the plaque. On average, 42% (ranging from 5 to 91%) of the sections from any single patient had C. pneumoniae DNA present. A patchy distribution of C. pneumoniae in the atherosclerotic lesions was observed, with no area of the carotid having significantly more C. pneumoniae DNA present. If a single random 30-?m-thick section was tested, there was only a 35.6 to 41.6% (95% confidence interval) chance of detecting C. pneumoniae DNA in a patient with carotid artery disease. A minimum of 15 sections would therefore be required to obtain a 95% chance of detecting all true positives. The low concentration and patchy distribution of C. pneumoniae DNA in atherosclerotic plaque appear to be among the reasons for inconsistency between laboratories in the results reported.
Details
- Title
- Distribution of Chlamydia pneumoniae DNA in atherosclerotic carotid arteries: Significance for sampling procedures
- Authors
- M Cochrane (Author) - Queensland University of TechnologyA Pospischil (Author) - University of QueenslandP Walker (Author) - University of QueenslandH Gibbs (Author) - Princess Alexandra HospitalPeter Timms (Author) - Queensland University of Technology
- Publication details
- Journal of Clinical Microbiology, Vol.41(4), pp.1454-1457
- Publisher
- American Society for Microbiology
- Date published
- 2003
- DOI
- 10.1128/JCM.41.4.1454-1457.2003
- ISSN
- 0095-1137
- Copyright note
- Copyright © 2003 American Society for Microbiology. Reproduced here in accordance with the publishers copyright policy.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449620702621
- Output Type
- Journal article
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