Discovery of a novel insulin-like peptide and insulin binding proteins in the Eastern rock lobster Sagmariasus verreauxi

Jennifer Chandler; Joseph Aizen; Abigail Elizur; Lian Hollander-Cohen; Stephen Battaglene; Tomer Ventura

doi:10.1016/j.ygcen.2014.08.018

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Discovery of a novel insulin-like peptide and insulin binding proteins in the Eastern rock lobster Sagmariasus verreauxi

Journal article

Peer reviewed

Discovery of a novel insulin-like peptide and insulin binding proteins in the Eastern rock lobster Sagmariasus verreauxi

Jennifer Chandler, Joseph Aizen, Abigail Elizur, Lian Hollander-Cohen, Stephen Battaglene and Tomer Ventura

General and Comparative Endocrinology, Vol.215, pp.76-87

2015

DOI: https://doi.org/10.1016/j.ygcen.2014.08.018

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Abstract

crustacea

insulin-like peptide (ILP)

drosophila ILP7 (DILP7)

insulin-like growth factor binding protein (IGFBP)

single-insulin binding domain (SIBD)

This study reports, for the first time in any of the commercially important decapod species, the identification of an insulin-like peptide (ILP), distinct from the androgenic gland hormone. Bioinformatics analysis of the de novo assembled spiny lobster, (Sagmariasus verreauxi) transcriptome, allowed identification of Sv-ILP1 as well as eight binding proteins. Binding proteins were termed as Sv-IGFBP, due to homology with the vertebrate insulin-like growth-factor binding protein and Sv-SIBD1-7, single insulin-binding domain protein (SIBD), similar to those identified in other invertebrate species. Sv-ILP1 was found to be expressed in the eyestalk, gonads and antennal gland of both sexes and to a lesser extent in male muscle, androgenic gland and hepatopancreas. The expression profiles of each binding protein were found to vary across tissues, with Sv-SIBD5, 6 and 7 showing higher expression in the gonad, demonstrated by PCR and digital gene expression. Further spatial investigations, using in-situ hybridisation, found Sv-ILP1 to be expressed in the neurosecretory cells of the thoracic ganglia, in keeping with the tissue expression of Drosophila ILP7 (DILP7). This correlative tissue expression, considered with the phylogenetic clustering of Sv-ILP1 and DILP7, suggests Sv-ILP1 to be a DILP7 orthologue. The broad expression of Sv-ILP1 strongly suggests that ILPs have a role beyond that of masculinisation in decapods. The function of these novel peptides may have application in enhancing aquaculture practices in the commercially important decapod species.

Details

Title: Discovery of a novel insulin-like peptide and insulin binding proteins in the Eastern rock lobster Sagmariasus verreauxi
Authors: Jennifer Chandler (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Joseph Aizen (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Lian Hollander-Cohen (Author) - Hebrew University of Jerusalem, Israel
Stephen Battaglene (Author) - University of Tasmania
Tomer Ventura (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: General and Comparative Endocrinology, Vol.215, pp.76-87
Publisher: Academic Press
Date published: 2015
DOI: 10.1016/j.ygcen.2014.08.018
ISSN: 0016-6480
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99448898602621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Endocrinology & Metabolism; Zoology

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