Journal article
Differential stabilities of soil enzymes. Assay and properties of phosphatase and arylsulphatase
Biochimica et Biophysica Acta (BBA) - Enzymology, Vol.485(2), pp.357-366
1977
Abstract
Methods have been refined for the assay of phosphatase and arylsulphatase activities in soil, based on the chromogenic p-nitrophenyl ester substrates. Basic assay conditions have been defined, and pH optima and kinetic parameters have been determined. The enzymes follow Michaelis-Menten kinetics; this conclusion is based on three methods of analysis of data determined over a wide range of substrate concentrations. The enzyme activities are very stable to storage of wet soil for up to 4 weeks at soil temperatures and above. For example, phosphatase had a half-life of approximately 2 weeks at 50°C; arylsulphatase was rather less stable. Both enzymes retained 80% of activity after incubation with pronase for 1 week at 25°C. On the basis of this work and studies on other soil enzymes, it is concluded that remarkable stability is a general feature of soil enzymes. © 1977.
Details
- Title
- Differential stabilities of soil enzymes. Assay and properties of phosphatase and arylsulphatase
- Authors
- N M Pettit (Author) - University of Kent, United KingdomL J Gregory (Author) - University of Kent, United KingdomR B Freedman (Author) - University of Kent, United KingdomRichard G Burns (Author) - University of Kent, United Kingdom
- Publication details
- Biochimica et Biophysica Acta (BBA) - Enzymology, Vol.485(2), pp.357-366
- Publisher
- Elsevier BV
- Date published
- 1977
- DOI
- 10.1016/0005-2744(77)90171-1
- ISSN
- 0005-2744
- Organisation Unit
- University of the Sunshine Coast, Queensland; Office of the Deputy Vice-Chancellor (Research and Innovation)
- Language
- English
- Record Identifier
- 99449595702621
- Output Type
- Journal article
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