Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes

Robert J Harvey; T Stuhmer; J Van Minnen; M G Darlison

doi:10.1002/(SICI)1520-6769(199701)20:1<31::AID-NRC183>3.0.CO;2-6

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Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes

Journal article

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Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes

Robert J Harvey, T Stuhmer, J Van Minnen and M G Darlison

Neuroscience Research Communications, Vol.20(1), pp.31-40

1997

DOI: https://doi.org/10.1002/(SICI)1520-6769(199701)20:1<31::AID-NRC183>3.0.CO;2-6

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Abstract

complementary DNA cloning

gene family

glutamate-gated cation channel

glutamate receptor

in situ hybridisation

mollusc (Lymnaea stagnalis)

We report the isolation of molluscan (Lymnaea stagnalis) partial complementary DNAs (cDNAs) that encode two new putative glutamate-gated cation-channel polypeptides. These proteins, which we have named Lym-eGluR4 and Lym-eGluR5, exhibit 56% identity to each other, and strong similarity (56% to 61%, and 56% to 68% identity, respectively) to the corresponding portions of vertebrate and invertebrate α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-selective (or AMPA-like) glutamate receptor (GluR) subunits. Using in situ hybridisation, we have shown that the Lym-eGluR4 and Lym-eGluR5 genes are both transcribed in all 11 central ganglia of the molluscan nervous system; however, their patterns of expression are different. Interestingly, the Lym-eGluR5 mRNA is present in an identifiable neuron (RPeD1), within the right pedal ganglion, that has recently been shown to possess a GluR that can be readily activated by L-glutamate, quisqualic acid and kainic acid.

Details

Title: Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes
Authors: Robert J Harvey (Author) - Universität Hamburg, Germany
T Stuhmer (Author) - Universität Hamburg, Germany
J Van Minnen (Author) - Vrije Universiteit, Netherlands
M G Darlison (Author) - Universität Hamburg, Germany
Publication details: Neuroscience Research Communications, Vol.20(1), pp.31-40
Publisher: John Wiley & Sons Ltd.
Date published: 1997
DOI: 10.1002/(SICI)1520-6769(199701)20:1<31::AID-NRC183>3.0.CO;2-6
ISSN: 0893-6609
Organisation Unit: School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450808002621
Output Type: Journal article

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