Differential expression of ompA, ompB, pyK, nlpD and Cpn0585 genes between normal and interferon-y, treated cultures of chlamydia pneumoniae

S Mathews; C George; C Flegg; D Stenzel; Peter Timms

doi:10.1006/mpat.2000.0435

Back

Differential expression of ompA, ompB, pyK, nlpD and Cpn0585 genes between normal and interferon-y, treated cultures of chlamydia pneumoniae

Journal article

Peer reviewed

Differential expression of ompA, ompB, pyK, nlpD and Cpn0585 genes between normal and interferon-y, treated cultures of chlamydia pneumoniae

S Mathews, C George, C Flegg, D Stenzel and Peter Timms

Microbial Pathogenesis, Vol.30(6), pp.337-345

2001

DOI: https://doi.org/10.1006/mpat.2000.0435

Files and links (1)

url

https://doi.org/10.1006/mpat.2000.0435View

Published Version

Abstract

chlamydia

persistence

chronic infection

gene regulation

interferon

A common feature of many chlamydial infections is that they are often asymptomatic and may persist for long periods of time if left untreated. In addition to the well recognized lytic stage of the chlamydial developmental cycle, evidence is now emerging to support a persistent phase in the cycle in which the reticulate bodies are morphologically abnormal, viable but non-infectious and presumably also have altered gene expression patterns. We used an RT-PCR approach to study the differential levels of gene transcription for 14 genes (16SrRNA, ompA, ompB, omcB, 76 kDa, gseA, pmp1, gltX, hsp60, yaeT, pyk, nlpD, Cpn0585, Cpn1046) between normal and IFN? treated Chlamydia pneumoniae cell cultures. Even though the level of morphologically abnormal reticulate bodies in our IFN-? treated cultures was low (approximately 10% morphologically discernible, although presumably a larger percentage were in the persistent state but not yet morphologically altered) we identified five genes (ompA, ompB, pyk, nlpD, Cpn0585) that were clearly upregulated when compared to normal cultures. This gene transcript profile may be characteristic of a general stress state in Chlamydia, induced by IFN-? treatment in this case, but perhaps more widely induced in other in vitro and in vivo situations. © 2001 Academic Press.

Details

Title: Differential expression of ompA, ompB, pyK, nlpD and Cpn0585 genes between normal and interferon-y, treated cultures of chlamydia pneumoniae
Authors: S Mathews (Author) - Queensland University of Technology
C George (Author) - Queensland University of Technology
C Flegg (Author) - Queensland University of Technology
D Stenzel (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
Publication details: Microbial Pathogenesis, Vol.30(6), pp.337-345
Publisher: Academic Press
Date published: 2001
DOI: 10.1006/mpat.2000.0435
ISSN: 0882-4010
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449418602621
Output Type: Journal article

Metrics

2 File views/ downloads

395 Record Views

50 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Web Of Science research areas: Immunology; Microbiology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites