Differential expression of neuropeptides correlates with growth rate in cultivated Haliotis asinina (Vetigastropoda: Mollusca)

P S York; Scott F Cummins; T Lucas; S P Blomberg; S M Degnan; B M Degnan

doi:10.1016/j.aquaculture.2011.12.019

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Differential expression of neuropeptides correlates with growth rate in cultivated Haliotis asinina (Vetigastropoda: Mollusca)

Journal article

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Differential expression of neuropeptides correlates with growth rate in cultivated Haliotis asinina (Vetigastropoda: Mollusca)

P S York, Scott F Cummins, T Lucas, S P Blomberg, S M Degnan and B M Degnan

Aquaculture, Vol.334-337, pp.159-168

2012

DOI: https://doi.org/10.1016/j.aquaculture.2011.12.019

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Abstract

gastropod

aquaculture

insulin

molluscan growth

hormone

tropical abalone

Rapid and efficient growth of animals in culture is critical to the success of most aquaculture industries. For example, the commercial cultivation of highly-prized abalone (Haliotidae: Vetigastropoda) has been hampered by naturally slow growth rates. To begin to investigate the molecular mechanisms controlling growth in haliotid molluscs, we chose to focus on neuropeptides secreted from anterior brain ganglia, because these have been implicated in controlling growth and reproduction in non-commercial gastropods. Here, we report the characterisation of three neuropeptides expressed in the anterior cerebral and pleuropedal ganglia of the tropical abalone Haliotis asinina; these are (1) the evolutionarily-conserved molluscan insulin-like peptide (Has-MIP), (2) a gastropod-specific schistosomin-like peptide (Has-SLP) and (3) a novel peptide currently known only from abalone, which we have called the haliotid growth-associated peptide (Has-HGAP). The novel Has-HGAP gene encodes a prepropeptide that undergoes substantial post-translational processing, including cleavage at an unusual site separating the two mature peptide chains of 19 and 51 amino acids in length; these are covalently bound by disulfide bonds. Quantitative comparison of Has-HGAP, Has-SLP and Has-MIP transcript abundance between the fastest and slowest growing individuals within a cohort of 6 month old cultured H. asinina reveals that Has-HGAP and Has-SLP are significantly upregulated in fast growing abalone; in contrast, Has-MIP is significantly downregulated. In light of their differential expression among juvenile abalone with markedly different growth rates, we suggest these neuropeptide genes will be fruitful points of focus for improving H. asinina growth rates in aquaculture by marker-assisted selective breeding.

Details

Title: Differential expression of neuropeptides correlates with growth rate in cultivated Haliotis asinina (Vetigastropoda: Mollusca)
Authors: P S York (Author) - University of Queensland
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
T Lucas (Author) - University of Queensland
S P Blomberg (Author) - University of Queensland
S M Degnan (Author) - University of Queensland
B M Degnan (Author) - University of Queensland
Publication details: Aquaculture, Vol.334-337, pp.159-168
Publisher: Elsevier BV
Date published: 2012
DOI: 10.1016/j.aquaculture.2011.12.019
ISSN: 0044-8486
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449510502621
Output Type: Journal article

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Web Of Science research areas: Fisheries; Marine & Freshwater Biology