Differential expression of genes encoding membrane proteins between acute and continuous Chlamydia pneumoniae infections

R J Hogan; S A Mathews; A Kutlin; M R Hammerschlag; Peter Timms

doi:10.1016/S0882-4010(02)00187-0

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Differential expression of genes encoding membrane proteins between acute and continuous Chlamydia pneumoniae infections

Journal article

Peer reviewed

Differential expression of genes encoding membrane proteins between acute and continuous Chlamydia pneumoniae infections

R J Hogan, S A Mathews, A Kutlin, M R Hammerschlag and Peter Timms

Microbial Pathogenesis, Vol.34(1), pp.11-16

2003

DOI: https://doi.org/10.1016/S0882-4010(02)00187-0

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Abstract

continuous infection

chlamydia pneumoniae

membrane protein

gene expression

real-time RT-PCR

persistence

Chlamydia pneumoniae is associated with several chronic human diseases, including chronic obstructive pulmonary disease and atherosclerotic cardiovascular disease. During chronic disease, organisms are believed to exist in a persistent phase that is not well understood at the genetic level. Long-term in vitro continuous infections are spontaneously persistent and are less susceptible than in vitro acute infections to treatment with antibiotics, and are therefore particularly relevant as an in vitro model of in vivo chronic disease. Real-time reverse transcriptase-PCR (r-t RT-PCR) was used to quantitate transcript copy numbers of 13 genes in continuous and acute infections with C. pneumoniae. The set of genes studied encodes proteins with known or predicted functions in the cell membrane, the inclusion membrane, cell division, metabolism, and immunopathology. Significant upregulation was seen for five genes (CPn0483, nlpD, ompA, pmp1 and porB) in continuous cultures. The genes omcB, pmp1, and porB, all of which encode membrane proteins, shared similar patterns of expression over both acute and continuous profiles. These results show that Chlamydia in the long-term continuous model of persistence have a unique transcription profile, adding to our knowledge of regulation of this important stage of chlamydial growth. Crown Copyright © 2003 Published by Elsevier Science Ltd. All rights reserved.

Details

Title: Differential expression of genes encoding membrane proteins between acute and continuous Chlamydia pneumoniae infections
Authors: R J Hogan (Author) - Queensland University of Technology
S A Mathews (Author) - Queensland University of Technology
A Kutlin (Author) - State University of New York Downstat Medical Centre, United States
M R Hammerschlag (Author) - State University of New York Downstat Medical Centre, United States
Peter Timms (Author) - Queensland University of Technology
Publication details: Microbial Pathogenesis, Vol.34(1), pp.11-16
Publisher: Academic Press
Date published: 2003
DOI: 10.1016/S0882-4010(02)00187-0
ISSN: 0882-4010
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449771502621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Immunology; Microbiology

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