Different and diverse anaerobic microbiota were seen in women living with HIV with unsuppressed HIV viral load and in women with recurrent bacterial vaginosis: a cohort study

C Elwood; A Y K Albert; E McClymont; E Wagner; D Mahal; K Devakandan; Bonnie L Quigley; Z Pakzad; M H Yudin; J E Hill; D Money; VOGUE Research Group

doi:10.1111/1471-0528.15930

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Different and diverse anaerobic microbiota were seen in women living with HIV with unsuppressed HIV viral load and in women with recurrent bacterial vaginosis: a cohort study

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Different and diverse anaerobic microbiota were seen in women living with HIV with unsuppressed HIV viral load and in women with recurrent bacterial vaginosis: a cohort study

C Elwood, A Y K Albert, E McClymont, E Wagner, D Mahal, K Devakandan, Bonnie L Quigley, Z Pakzad, M H Yudin, J E Hill, …

BJOG : an international journal of obstetrics and gynaecology, Vol.127(2), pp.250-259

2020

DOI: https://doi.org/10.1111/1471-0528.15930

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Abstract

bacterial vaginosis

cpn60

HIV

vaginal microbiome

Objective To compare the vaginal microbiota of women living with HIV (WLWH) with the vaginal microbiota of women with recurrent bacterial vaginosis (BV) and healthy women without HIV to determine if there are differences in the vaginal microbiome, what factors influence these differences, and to characterise HIV clinical parameters including viral load and CD4 count in relation to the vaginal microbiome. Design Observational cohort study. Setting Canada. Population Women aged 18-49 years who were premenopausal and not pregnant were recruited into three cohorts: healthy women, WLWH and women with recurrent BV. Methods Demographic and clinical data were collected via interviews and medical chart reviews. Vaginal swabs were collected for Gram-stain assessment and microbiome profiling using the cpn60 barcode sequence. Main outcome measures To compare overall community composition differences, we used compositional data analysis methods, hierarchical clustering and Kruskal-Wallis tests where appropriate. Results Clinical markers such as odour and abnormal discharge, but not irritation, were associated with higher microbial diversity. WLWH with unsuppressed HIV viral loads were more likely than other groups to have non-Gardnerella-dominated microbiomes. HIV was associated with higher vaginal microbial diversity and this was related to HIV viral load, with unsuppressed women demonstrating significantly higher relative abundance of Megasphaera genomosp. 1, Atopobium vaginae and Clostridiales sp. (all P < 0.05) compared with all other groups. Conclusions In WLWH, unsuppressed HIV viral loads were associated with a distinct dysbiotic profile consisting of very low levels of Lactobacillus and high levels of anaerobes.

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Title: Different and diverse anaerobic microbiota were seen in women living with HIV with unsuppressed HIV viral load and in women with recurrent bacterial vaginosis: a cohort study
Authors: C Elwood (Author) - University of British Columbia, Canada
A Y K Albert (Author) - Women's Health Research Institute, Canada
E McClymont (Author) - University of British Columbia, Canada
E Wagner (Author) - Women's Health Research Institute, Canada
D Mahal (Author) - Women's Health Research Institute, Canada
K Devakandan (Author) - University of British Columbia, Canada
Bonnie L Quigley (Author) - University of Saskatchewan, Canada
Z Pakzad (Author) - Women's Health Research Institute, Canada
M H Yudin (Author) - University of Toronto, Canada
J E Hill (Author) - University of Saskatchewan, Canada
D Money (Corresponding Author) - University of British Columbia, Canada
VOGUE Research Group (Author)
Publication details: BJOG : an international journal of obstetrics and gynaecology, Vol.127(2), pp.250-259
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2020
DOI: 10.1111/1471-0528.15930
ISSN: 1470-0328; 1470-0328
Copyright note: Copyright (c) 2020. This is the peer reviewed version of the following article: Elwood, C, Albert, AYK, McClymont, E, Wagner, E, Mahal, D, Devakandan, K, Quiqley, BL, Pakzad, Z, Yudin, MH, Hill, JE, Money, D, the VOGUE Research Group. Different and diverse anaerobic microbiota were seen in women living with HIV with unsuppressed HIV viral load and in women with recurrent bacterial vaginosis: a cohort study. BJOG 2020; 127: 250– 259, which has been published in final form at http://dx.doi.org/10.1111/1471-0528.15930. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions
Organisation Unit: University of the Sunshine Coast, Queensland; Thompson Institute; Centre for Bioinnovation
Language: English
Record Identifier: 99450639602621
Output Type: Journal article
Research Statement: false

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