Journal article
Differences in virulence repertoire and cell invasive potential of Group A Streptococcus emm1-2 in comparison to emm1 genotype
International Journal of Medical Microbiology, Vol.304(5-6), pp.685-695
2014
Abstract
Group A streptococcus (GAS, Streptococcus pyogenes) type emm1 is widely associated with streptococcal invasive disease. This type is prevalent worldwide but is rare in India. Instead, emm1-2 type which is closely related to emm1 but is a distinct type is more prevalent. Although emm1 has been well characterized, information available on emm1-2 is rare. In this study we present a comparative study of both types. DNA microarray analysis showed segregation of emm1 and emm1-2 isolates into two distinct clusters. Out of 229 arrayed genes, 83-87% were present, 6-9% absent and 4-8% genes were ambiguous in emm1 isolates. emm1-2 strains harbored only 68-77%, 11-13% were absent and 10-20% ambiguous genes. Fourteen genes, present in all emm1, were completely absent in the emm1-2 isolates. sfb1 is a gene which encodes for Streptococcal fibronectin binding adhesin and invasin which has restricted distribution among different emm types of GAS. A variant of sfb1 (sfb1-2) was the only gene which was present in all emm1-2 isolates, but absent from all emm1 strains. Sfb1 and Sfb1-2 differ in sequences in the aromatic domain and the proline rich repeat region, whereas the fibronectin binding region was conserved and exhibited similar fibronectin binding activity. The presence of Sfb1-2 in emm1-2 strains was concomitant with significantly higher fibronectin-binding and invasion efficiency of HEp-2 cells when compared to emm1 isolates. The role of Sfb1-2 in invasion was confirmed by latex bead assay. emm1-2 isolates follow membrane ruffling mechanism during invasion and intracellularly follow classical endocytic pathway. Further studies are required to understand the correlation between the presence of emm1-2 isolates and the disease pattern in North India.
Details
- Title
- Differences in virulence repertoire and cell invasive potential of Group A Streptococcus emm1-2 in comparison to emm1 genotype
- Authors
- Vivek Sagar (Author) - Helmholtz Centre for Infection Research, GermanyRene Bergmann (Author) - Helmholtz Centre for Infection Research, GermanyAndreas Nerlich (Author) - Helmholtz Centre for Infection Research, GermanyDavid J McMillan (Author) - Queensland Institute of Medical ResearchD Patric Nitsch-Schmitz (Author) - Helmholtz Centre for Infection Research, GermanyMarcus Fulde (Author) - Helmholtz Centre for Infection ResearchSusanne Talay (Author) - Helmholtz Centre for Infection ResearchRobert Geffers (Author) - Helmholtz Centre for Infection ResearchNancy Hoe (Author) - National Institute of HealthRajesh Kumar (Author) - Postgraduate Institute of Medical Education and ResearchManfred Rohde (Author) - Helmholtz Centre for Infection ResearchAnuradha Chakroborti (Author) - Postgraduate Institute of Medical Education and ResearchGursharan S Chhatwal (Author) - Helmholtz Centre for Infection Research
- Publication details
- International Journal of Medical Microbiology, Vol.304(5-6), pp.685-695
- Publisher
- Urban & Fischer Verlag
- Date published
- 2014
- DOI
- 10.1016/j.ijmm.2014.04.011
- ISSN
- 1438-4221
- Copyright note
- Copyright © 2014. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99448780002621
- Output Type
- Journal article
Metrics
102 File views/ downloads
1690 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Microbiology
- Virology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites