Journal article
Dietary Supplementation with Omega-3 Polyunsaturated Fatty Acids Modulate Matrix Metalloproteinase Immunoreactivity in a Mouse Model of Pre-abdominal Aortic Aneurysm
Heart, Lung and Circulation, Vol.24(4), pp.377-385
2015
Abstract
Background: Two-day infusion of angiotensin II to apolipoprotein E-deficient (ApoE-/-) mice provides a model of pre-abdominal aortic aneurysm. Long chain omega-3 polyunsaturated fatty acids (n-3 PUFAs) have anti-inflammatory effects. This study examined the effect of an eight-week low or high n-3 PUFA diet in ApoE-/- mice on matrix metalloproteinase (MMP) expression and elastin degradation. Methods: ApoE-/- mice were fed a low or high n-3 PUFA diet for eight weeks prior to two-day infusion with angiotensin II. The omega-3 index, MMP-2, MMP-9, TIMP-1, and TGF-β1 immunoreactivity, and elastin fragmentation were measured. Results: The omega-3 index with the low and high n-3 PUFA diet was 3.78% and 13.03%, respectively. MMP-9 immunoreactive stain intensity was lower in mice fed the high, compared to the low n-3 PUFA diet in endothelial cells (suprarenal aorta), and inflammatory cells (suprarenal and infrarenal aorta). Inflammatory cells had higher TIMP-1 and TGF-β1 stain intensity in mice fed the high, compared to the low n-3 PUFA diet (suprarenal aorta). MMP-2 immunoreactivity was unaffected by diet. A non-significant trend for reduced elastin fragmentation was observed in mice fed the high n-3 PUFA diet. Conclusion: Dietary supplementation with n-3 PUFAs may have protective anti-inflammatory effects mediated through modulation of MMPs and TIMPs.
Details
- Title
- Dietary Supplementation with Omega-3 Polyunsaturated Fatty Acids Modulate Matrix Metalloproteinase Immunoreactivity in a Mouse Model of Pre-abdominal Aortic Aneurysm
- Authors
- Kristyn Kavazos (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMaria Nataatmadja (Author) - University of QueenslandKathryn Wales (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringElke Hartland (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringChloe Williams (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringFraser D Russell (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Heart, Lung and Circulation, Vol.24(4), pp.377-385
- Publisher
- Elsevier Australia
- Date published
- 2015
- DOI
- 10.1016/j.hlc.2014.11.005
- ISSN
- 1443-9506
- Copyright note
- Copyright © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Health - Biomedicine; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99448722302621
- Output Type
- Journal article
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