Development of ligase-assisted spacer addition for the measurement of microsatellites

V Brockhurst; R Barnard; L Wolter; P Giffard; Peter Timms

doi:10.2144/01311rr01

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Development of ligase-assisted spacer addition for the measurement of microsatellites

Journal article

Peer reviewed

Development of ligase-assisted spacer addition for the measurement of microsatellites

V Brockhurst, R Barnard, L Wolter, P Giffard and Peter Timms

BioTechniques, Vol.31(1), pp.96-106

2001

DOI: https://doi.org/10.2144/01311rr01

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Abstract

Conventional methods for detecting differences in microsatellite repeat lengths rely on electrophoretic fractionation on long denaturing polyacrylamide gels, a time-consuming and labor-intensive method. Therefore, there is a need for the development of new and rapid approaches to routinely detect such length polymorphisms. The advent of techniques allowing the coupling of DNA molecules to solid surfaces has provided new prospects in the area of mutation detection. We describe here the development and optimization of the ligase-assisted spacer addition (LASA) method, a novel and rapid procedure based on an ELISA format to measure microsatellite repeat lengths. The LASA assay was successfully applied to a set of 11 bird samples to assess its capabilities as a genotyping method.

Details

Title: Development of ligase-assisted spacer addition for the measurement of microsatellites
Authors: V Brockhurst (Author) - Queensland University of Technology
R Barnard (Author) - Queensland University of Technology
L Wolter (Author) - Queensland University of Technology
P Giffard (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
Publication details: BioTechniques, Vol.31(1), pp.96-106
Publisher: Informa Healthcare
DOI: 10.2144/01311rr01
ISSN: 0736-6205
Organisation Unit: Centre for Bioinnovation; University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99449755002621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemical Research Methods; Biochemistry & Molecular Biology

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