Journal article
Development of ligase-assisted spacer addition for the measurement of microsatellites
BioTechniques, Vol.31(1), pp.96-106
2001
Abstract
Conventional methods for detecting differences in microsatellite repeat lengths rely on electrophoretic fractionation on long denaturing polyacrylamide gels, a time-consuming and labor-intensive method. Therefore, there is a need for the development of new and rapid approaches to routinely detect such length polymorphisms. The advent of techniques allowing the coupling of DNA molecules to solid surfaces has provided new prospects in the area of mutation detection. We describe here the development and optimization of the ligase-assisted spacer addition (LASA) method, a novel and rapid procedure based on an ELISA format to measure microsatellite repeat lengths. The LASA assay was successfully applied to a set of 11 bird samples to assess its capabilities as a genotyping method.
Details
- Title
- Development of ligase-assisted spacer addition for the measurement of microsatellites
- Authors
- V Brockhurst (Author) - Queensland University of TechnologyR Barnard (Author) - Queensland University of TechnologyL Wolter (Author) - Queensland University of TechnologyP Giffard (Author) - Queensland University of TechnologyPeter Timms (Author) - Queensland University of Technology
- Publication details
- BioTechniques, Vol.31(1), pp.96-106
- Publisher
- Informa Healthcare
- Date published
- 2001
- DOI
- 10.2144/01311rr01
- ISSN
- 0736-6205
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449755002621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Web Of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology