Journal article
Development of ceftazidime resistance in an acute burkholderia pseudomallei infection
Infection and Drug Resistance, Vol.5(1), pp.129-132
2012
Abstract
Burkholderia pseudomallei, a bacterium that causes the disease melioidosis, is intrinsically resistant to many antibiotics. First-line antibiotic therapy for treating melioidosis is usually the synthetic β-lactam, ceftazidime (CAZ), as almost all B. pseudomallei strains are susceptible to this drug. However, acquired CAZ resistance can develop in vivo during treat- ment with CAZ, which can lead to mortality if therapy is not switched to a different drug in a timely manner. Serial B. pseudomallei isolates obtained from an acute Thai melioidosis patient infected by a CAZ susceptible strain, who ultimately succumbed to infection despite being on CAZ therapy for the duration of their infection, were analyzed. Isolates that developed CAZ resistance due to a proline to serine change at position 167 in the β-lactamase PenA were identi- fed. Importantly, these CAZ resistant isolates remained sensitive to the alternative melioidosis treatments; namely, amoxicillin-clavulanate, imipenem, and meropenem. Lastly, real-time polymerase chain reaction-based assays capable of rapidly identifying CAZ resistance in B. pseudomallei isolates at the position 167 mutation site were developed. The ability to rapidly identify the emergence of CAZ resistant B. pseudomallei populations in melioidosis patients will allow timely alterations in treatment strategies, thereby improving patient outcomes for this serious disease. © 2012 Sarovich et al, publisher and licensee Dove Medical Press Ltd.
Details
- Title
- Development of ceftazidime resistance in an acute burkholderia pseudomallei infection
- Authors
- Derek S Sarovich (Author) - Northern Arizona University, United StatesErin P Price (Author) - Northern Arizona University, United StatesD Limmathurotsakul (Author) - Mahidol University, ThailandJ M Cook (Author) - Northern Arizona University, United StatesA T von Schulze (Author) - Northern Arizona University, United StatesS R Wolken (Author) - Northern Arizona University, United StatesP Keim (Author) - Northern Arizona University, United StatesSharon J Peacock (Author) - Mahidol University, ThailandT Pearson (Author) - Northern Arizona University, United States
- Publication details
- Infection and Drug Resistance, Vol.5(1), pp.129-132
- Publisher
- Dove Medical Press Ltd.
- Date published
- 2012
- DOI
- 10.2147/IDR.S35529
- ISSN
- 1178-6973; 1178-6973
- Copyright note
- Copyright © 2012 The Authors. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450316202621
- Output Type
- Journal article
- Research Statement
- false
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