Development of a soybean leaf disc assay for determining oral insecticidal activity in the lepidopteran agricultural pest Helicoverpa armigera

Yachen Wang; Shaodong Guo; Tomer Ventura; Ritesh Jain; Karl E Robinson; Neena Mitter; Volker Herzig

doi:10.1016/j.toxicon.2023.107588

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Development of a soybean leaf disc assay for determining oral insecticidal activity in the lepidopteran agricultural pest Helicoverpa armigera

Journal article

Open access

Peer reviewed

Development of a soybean leaf disc assay for determining oral insecticidal activity in the lepidopteran agricultural pest Helicoverpa armigera

Yachen Wang, Shaodong Guo, Tomer Ventura, Ritesh Jain, Karl E Robinson, Neena Mitter and Volker Herzig

Toxicon, Vol.238, pp.1-7

2024

DOI: https://doi.org/10.1016/j.toxicon.2023.107588

PMID: 38147939

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Abstract

Screening

Orally insecticidal activity

Arachnid venom

Imidacloprid

Bt toxin

Lepidopteran

Pest insects pose a heavy burden on global agricultural industries with small molecule insecticides being predominantly used for their control. Unwanted side effects and resistance development plagues most small molecule insecticides such as the neonicotinoids, which have been reported to be harmful to honeybees. Bioinsecticides like Bacillus thuringiensis (Bt) toxins can be used as environmentally-friendly alternatives. Arachnid venoms comprise another promising source of bioinsecticides, containing a multitude of selective and potent insecticidal toxins. Unfortunately, no standardised insect models are currently available to assess the suitability of insecticidal agents under laboratory conditions. Thus, we aimed to develop a laboratory model that closely mimics field conditions by employing a leaf disk assay (LDA) for oral application of insecticidal agents in a bioassay tray format. Neonate larvae of the cotton bollworm (Helicoverpa armigera) were fed with soybean (Glycine max) leaves that were treated with different insecticidal agents. We observed dose-dependent insecticidal effects for Bt toxin and the neonicotinoid insecticide imidacloprid, with imidacloprid exhibiting a faster response. Furthermore, we identified several insecticidal arachnid venoms that were active when co-applied with sub-lethal doses of Bt toxin. We propose the H. armigera LDA as a suitable tool for assessing the insecticidal effects of insecticidal agents against lepidopterans.

Details

Title: Development of a soybean leaf disc assay for determining oral insecticidal activity in the lepidopteran agricultural pest Helicoverpa armigera
Authors: Yachen Wang (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Shaodong Guo (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Tomer Ventura (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Ritesh Jain (Author) - The University of Queensland
Karl E Robinson (Author) - The University of Queensland
Neena Mitter (Author) - The University of Queensland
Volker Herzig (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Publication details: Toxicon, Vol.238, pp.1-7
Publisher: Elsevier Ltd
Date published: 2024
DOI: 10.1016/j.toxicon.2023.107588
ISSN: 1879-3150
PMID: 38147939
Data Availability: Data will be made available on request.
Grants: Be(e) friendly venomous spiders! Novel biopesticides from arachnid venoms, FT190100482, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99986198902621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Pharmacology & Pharmacy; Toxicology

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