Journal article
Development of a soybean leaf disc assay for determining oral insecticidal activity in the lepidopteran agricultural pest Helicoverpa armigera
Toxicon, Vol.238, pp.1-7
2024
PMID: 38147939
Abstract
Pest insects pose a heavy burden on global agricultural industries with small molecule insecticides being predominantly used for their control. Unwanted side effects and resistance development plagues most small molecule insecticides such as the neonicotinoids, which have been reported to be harmful to honeybees. Bioinsecticides like Bacillus thuringiensis (Bt) toxins can be used as environmentally-friendly alternatives. Arachnid venoms comprise another promising source of bioinsecticides, containing a multitude of selective and potent insecticidal toxins. Unfortunately, no standardised insect models are currently available to assess the suitability of insecticidal agents under laboratory conditions. Thus, we aimed to develop a laboratory model that closely mimics field conditions by employing a leaf disk assay (LDA) for oral application of insecticidal agents in a bioassay tray format. Neonate larvae of the cotton bollworm (Helicoverpa armigera) were fed with soybean (Glycine max) leaves that were treated with different insecticidal agents. We observed dose-dependent insecticidal effects for Bt toxin and the neonicotinoid insecticide imidacloprid, with imidacloprid exhibiting a faster response. Furthermore, we identified several insecticidal arachnid venoms that were active when co-applied with sub-lethal doses of Bt toxin. We propose the H. armigera LDA as a suitable tool for assessing the insecticidal effects of insecticidal agents against lepidopterans.
Details
- Title
- Development of a soybean leaf disc assay for determining oral insecticidal activity in the lepidopteran agricultural pest Helicoverpa armigera
- Authors
- Yachen Wang (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationShaodong Guo (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationTomer Ventura (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationRitesh Jain (Author) - The University of QueenslandKarl E Robinson (Author) - The University of QueenslandNeena Mitter (Author) - The University of QueenslandVolker Herzig (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- Toxicon, Vol.238, pp.1-7
- Publisher
- Elsevier Ltd
- Date published
- 2024
- DOI
- 10.1016/j.toxicon.2023.107588
- ISSN
- 1879-3150
- PMID
- 38147939
- Data Availability
- Data will be made available on request.
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99986198902621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Pharmacology & Pharmacy
- Toxicology
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