Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper

Lachlan Dennis; Josephine Nocillado; Peter Palma; Takafumi Amagai; Kiyoshi Soyano; Abigail Elizur

doi:10.1016/j.ygcen.2020.113542

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Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper

Journal article

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Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper

Lachlan Dennis, Josephine Nocillado, Peter Palma, Takafumi Amagai, Kiyoshi Soyano and Abigail Elizur

General and Comparative Endocrinology, Vol.296, pp.1-12

2020

DOI: https://doi.org/10.1016/j.ygcen.2020.113542

PMID: 32585213

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Abstract

luteinizing hormone

grouper

ELISA

reproductive biology

A recombinant giant grouper Luteinizing Hormone (LH) consisting of tethered beta and alpha subunits was produced in a yeast expression system. The giant grouper LH β-subunit was also produced and administered to rabbits for antibody development. The recombinant LH and its antibody were used to develop an Enzyme Linked Immunosorbent Assay (ELISA). This ELISA enabled detection of plasma LH levels in groupers at a sensitivity between 391 pg/ml and 200 ng/ml. Different species of grouper were assayed with this ELISA in conjunction with gonadal histology and body condition data to identify links between circulating LH levels and sexual development. We found that circulating levels of LH decreased when oocytes began to degenerate, and sex-transition gonadal characteristics were apparent when LH levels decreased further. When circulating LH levels were related to body condition (body weight/ body length), transitioning-stage fish had relatively high body condition but low plasma LH levels. This observation was similar across multiple grouper species and indicates that plasma LH levels combined with body condition may be a marker for early male identification in the protogynous hermaphrodite groupers.

Details

Title: Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper
Authors: Lachlan Dennis (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Josephine Nocillado (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Peter Palma (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Takafumi Amagai (Author) - Nagasaki University
Kiyoshi Soyano (Author) - Nagasaki University
Abigail Elizur (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Publication details: General and Comparative Endocrinology, Vol.296, pp.1-12
Publisher: Elsevier Inc.
Date published: 2020
DOI: 10.1016/j.ygcen.2020.113542
ISSN: 1095-6840
PMID: 32585213
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99462008202621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Endocrinology & Metabolism; Zoology

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