Journal article
Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper
General and Comparative Endocrinology, Vol.296, pp.1-12
2020
Abstract
A recombinant giant grouper Luteinizing Hormone (LH) consisting of tethered beta and alpha subunits was produced in a yeast expression system. The giant grouper LH β-subunit was also produced and administered to rabbits for antibody development. The recombinant LH and its antibody were used to develop an Enzyme Linked Immunosorbent Assay (ELISA). This ELISA enabled detection of plasma LH levels in groupers at a sensitivity between 391 pg/ml and 200 ng/ml. Different species of grouper were assayed with this ELISA in conjunction with gonadal histology and body condition data to identify links between circulating LH levels and sexual development. We found that circulating levels of LH decreased when oocytes began to degenerate, and sex-transition gonadal characteristics were apparent when LH levels decreased further. When circulating LH levels were related to body condition (body weight/ body length), transitioning-stage fish had relatively high body condition but low plasma LH levels. This observation was similar across multiple grouper species and indicates that plasma LH levels combined with body condition may be a marker for early male identification in the protogynous hermaphrodite groupers.
Details
- Title
- Development of a giant grouper Luteinizing Hormone (LH) Enzyme-Linked Immunosorbent Assay (ELISA) and its use towards understanding sexual development in grouper
- Authors
- Lachlan Dennis (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyJosephine Nocillado (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyPeter Palma (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyTakafumi Amagai (Author) - Nagasaki UniversityKiyoshi Soyano (Author) - Nagasaki UniversityAbigail Elizur (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
- Publication details
- General and Comparative Endocrinology, Vol.296, pp.1-12
- Publisher
- Elsevier Inc.
- DOI
- 10.1016/j.ygcen.2020.113542
- ISSN
- 1095-6840
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
- Language
- English
- Record Identifier
- 99462008202621
- Output Type
- Journal article
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- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Endocrinology & Metabolism
- Zoology
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