Journal article
Development of High-Throughput Fluorescent-Based Screens to Accelerate Discovery of P2X Inhibitors from Animal Venoms
Journal of Natural Products, Vol.82, pp.2559-2567
2019
Abstract
Animal venoms can play an important role in drug discovery, as they are a rich source of evolutionarily tuned compounds that target a variety of ion channels and receptors. To date, there are six FDA-approved drugs derived from animal venoms, with recent work using high-throughput platforms providing a variety of new therapeutic candidates. However, high-throughput methods for screening animal venoms against purinoceptors, one of the oldest signaling receptor families, have not been reported. Here, we describe a variety of quantitative fluorescent-based high-throughput screening (HTS) cell-based assays for screening animal venoms against ligand-gated P2X receptors. A diverse selection of 180 venoms from arachnids, centipedes, hymenopterans, and cone snails were screened, analyzed, and validated, both analytically and pharmacologically. Using this approach, we performed screens against human P2X3, P2X4, and P2X7 using three different fluorescent-based dyes on stable cell lines and isolated the active venom components. Our HTS assays are performed in 96-well format and allow simultaneous screening of multiple venoms on multiple targets, improving testing characteristics while minimizing costs, specimen material, and testing time. Moreover, utilizing our assays and applying them to the other natural product libraries, rather than venoms, might yield other novel natural products that modulate P2X activity.
Details
- Title
- Development of High-Throughput Fluorescent-Based Screens to Accelerate Discovery of P2X Inhibitors from Animal Venoms
- Authors
- L Bibic (Author) - University of East Anglia, United KingdomVolker Herzig (Author) - University of QueenslandG F King (Author) - University of QueenslandL Stokes (Author) - University of East Anglia, United Kingdom
- Publication details
- Journal of Natural Products, Vol.82, pp.2559-2567
- Publisher
- American Chemical Society
- DOI
- 10.1021/acs.jnatprod.9b00410
- ISSN
- 0163-3864
- Organisation Unit
- School of Science, Technology and Engineering; University of the Sunshine Coast, Queensland; School of Science and Engineering - Legacy; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450964502621
- Output Type
- Journal article
Metrics
34 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Chemistry, Medicinal
- Pharmacology & Pharmacy
- Plant Sciences
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites