Journal article
Development and function of pearl-sacs grown from regenerated mantle graft tissue in the black-lip pearl oyster, Pinctada margaritifera (Linnaeus, 1758)
Fish and Shellfish Immunology, Vol.45(2), pp.567-573
2015
Abstract
Current pearl grafting techniques were developed in the early 1900s and have changed little since. They involve the sacrifice of donor pearl oysters to provide graft tissue (saibo) that is implanted into host oysters. This study assessed the feasibility of using regenerated graft tissue for pearl production in the 'black-lip' pearl oyster, Pinctada margaritifera. Twelve days after grafting with regenerated graft tissue, there was complete encapsulation of the nucleus by the fully developed pearl-sac and the first layer of organic matrix had been secreted. Sixteen days after grafting, the pearl-sac was completely integrated with host tissue. The epithelial cells in the pearl-sac continued to secrete the organic matrix layer but there were no signs of nacre deposition at this stage. However, after three months of culture, nuclei in oysters grafted with regenerated mantle tissue were completely covered with nacre. The average nacre thickness on pearls produced from regenerated (0.547±0.01 mm, n = 8) and normal (0.532±0.01 mm, n = 8) mantle tissue did not differ significantly (p > 0.05). Nacre secretion rates, over the 80 day period subsequent to pearl-sac formation were 6.84±0.1 μm day-1 and 6.66±0.1 μm day-1 for oysters grafted with regenerated and normal mantle tissue, respectively. These means were not significantly different (p = 0.258). Our results clearly show that regenerated mantle tissue can function successfully as saibo for pearl production in P. margaritifera. This finding could provide significant benefits to pearl farmers and a basis for further development of current pearl grafting practices.
Details
- Title
- Development and function of pearl-sacs grown from regenerated mantle graft tissue in the black-lip pearl oyster, Pinctada margaritifera (Linnaeus, 1758)
- Authors
- Pranesh Kishore (Author) - James Cook UniversityPaul C Southgate (Author) - James Cook University
- Publication details
- Fish and Shellfish Immunology, Vol.45(2), pp.567-573
- Publisher
- Academic Press
- Date published
- 2015
- DOI
- 10.1016/j.fsi.2015.05.008
- ISSN
- 1050-4648
- Organisation Unit
- School of Science and Engineering - Legacy; Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99449552202621
- Output Type
- Journal article
Metrics
4 File views/ downloads
795 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Fisheries
- Immunology
- Marine & Freshwater Biology
- Veterinary Sciences
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites