Development and evaluation of a multi-antigen peptide ELISA for the diagnosis of Chlamydia trachomatis related infertility in women

Shruti Menon; Scott H Stansfield; Benignus Logan; Jane S Hocking; Peter Timms; Luk Rombauts; John A Allan; Wilhelmina May Huston

doi:10.1099/jmm.0.000311

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Development and evaluation of a multi-antigen peptide ELISA for the diagnosis of Chlamydia trachomatis related infertility in women

Journal article

Open access

Peer reviewed

Development and evaluation of a multi-antigen peptide ELISA for the diagnosis of Chlamydia trachomatis related infertility in women

Shruti Menon, Scott H Stansfield, Benignus Logan, Jane S Hocking, Peter Timms, Luk Rombauts, John A Allan and Wilhelmina May Huston

Journal of Medical Microbiology, Vol.65(9), pp.915-922

2016

DOI: https://doi.org/10.1099/jmm.0.000311

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Abstract

chlamydia

serology

tubal factor

ELISA

peptide

Chlamydia trachomatis results in tubal factor infertility in some women. Diagnosis of this tubal infertility is difficult and typically involves laparoscopy or hysterosalpingography to detect the tubal blockages. Numerous serological tests have been developed however are presently not used for diagnosis without subsequent surgical during the infertility investigation. This study aimed to develop a highly specific serological assay for chlamydial tubal factor infertility in women that could be used to recommend direct progression to IVF treatment for women who are positive. Women were recruited from a variety of settings including; women seeking fertility treatment, sexual health and GP consults, or antenatal care (n=259). The serological assay was developed using sera from a large group of women by using infertile MIF positive women with tubal damage as the positives compared to infertile, or acute infection, and/or fertile controls (negatives). The new multi-peptide ELISA was highly specific for the detection of tubal factor infertility (p=0.011) compared to another ELISA (p=0.022) and MIF (p=0.099). The sensitivity of the assay should be improved before clinical utility. Potentially a two-step testing protocol could be used during the initial infertility investigation where MIF followed by a highly specific ELISA could be used to recommend direct progression to IVF for women who are positive.

Details

Title: Development and evaluation of a multi-antigen peptide ELISA for the diagnosis of Chlamydia trachomatis related infertility in women
Authors: Shruti Menon (Author) - Queensland University of Technology
Scott H Stansfield (Author) - Queensland University of Technology
Benignus Logan (Author) - UC Health Clinical School
Jane S Hocking (Author) - University of Melbourne
Peter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Luk Rombauts (Author) - MIMR-PH Institute of Medical Research
John A Allan (Author) - Wesley Hospital
Wilhelmina May Huston (Author) - University of Technology Sydney
Publication details: Journal of Medical Microbiology, Vol.65(9), pp.915-922
Publisher: Microbiology Society
Date published: 2016
DOI: 10.1099/jmm.0.000311
ISSN: 0022-2615
Copyright note: Copyright © 2016 The Author. The author's accepted version is reproduced here in accordance with the publisher's copyright policy. The definitive version is available at: http://dx.doi.org/10.1099/jmm.0.000311
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99450469002621
Output Type: Journal article

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