Journal article
Development and characterization of microsatellite loci for Ocotea species (Lauraceae) threatened with extinction
Genetics and Molecular Research, Vol.13(3), pp.5138-5142
2014
Abstract
The Atlantic rainforest species Ocotea catharinensis, Ocotea odorifera, and Ocotea porosa have been extensively harvested in the past for timber and oil extraction and are currently listed as threatened due to overexploitation. To investigate the genetic diversity and population structure of these species, we developed 8 polymorphic microsatellite markers for O. odorifera from an enriched microsatellite library by using 2 dinucleotide repeats. The microsatellite markers were tested for cross-amplification in O. catharinensis and O. porosa. The average number of alleles per locus was 10.2, considering all loci over 2 populations of O. odorifera. Observed and expected heterozygosities for O. odorifera ranged from 0.39 to 0.93 and 0.41 to 0.92 across populations, respectively. Cross-amplification of all loci was successfully observed in O. catharinensis and O. porosa except 1 locus that was found to lack polymorphism in O. porosa. Combined probabilities of identity in the studied Ocotea species were very low ranging from 1.0 x 10-24 to 7.7 x 10-24. The probability of exclusion over all loci estimated for O. odorifera indicated a 99.9% chance of correctly excluding a random nonparent individual. The microsatellite markers described in this study have high information content and will be useful for further investigations on genetic diversity within these species and for subsequent conservation purposes.
Details
- Title
- Development and characterization of microsatellite loci for Ocotea species (Lauraceae) threatened with extinction
- Authors
- E M Martins (Author) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, BrasilG Martinelli (Author) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, BrasilM P Arbetman (Author) - Universidad Nacional de Río Negro, ArgentinaRobert W Lamont (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringJ L Simoes-Araujo (Author) - Empresa Brasileira de Pesquisa Agropecuária, BrasilDaniel Powell (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringM Ciampi-Guillardi (Author) - Universidade Estadual de Campinas, BrasilC Baldauf (Author) - Universidade Federal Rural do Semi-árido, BrasilA Quinet (Author) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, BrasilP Galisa (Author) - Universidade Federal Rural do Rio de Janeiro, BrasilAlison Shapcott (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Genetics and Molecular Research, Vol.13(3), pp.5138-5142
- Publisher
- Fundacao de Pesquisas Cientificas de Ribeirao Preto
- Date published
- 2014
- DOI
- 10.4238/2014.July.7.6
- ISSN
- 1676-5680
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; Office of Research; School of Science, Technology and Engineering; School of Law and Society; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99448939802621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Genetics & Heredity