Development and characterization of microsatellite loci for Khaya senegalensis (Meliaceae)

G J Sexton; Celine H Frere; M J Dieters; I D Godwin; P J Prentis

doi:10.3732/ajb.1000300

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Development and characterization of microsatellite loci for Khaya senegalensis (Meliaceae)

Journal article

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Development and characterization of microsatellite loci for Khaya senegalensis (Meliaceae)

G J Sexton, Celine H Frere, M J Dieters, I D Godwin and P J Prentis

American Journal of Botany, Vol.97(11), pp.e111-e113

2010

DOI: https://doi.org/10.3732/ajb.1000300

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Abstract

conservation genetics

Khaya senegalensis

microsatellite

next-generation sequencing

Premise of the study: Microsatellite loci were developed to characterize genetic variation and population subdivision in Khaya senegalensis (Desr.) A. Juss. (Meliaceae).Methods and Results: Microsatellite loci were identifi ed from genomic DNA sequences generated using the 454 GS-FLX titanium platform. Primers were designed for 67 tri- and tetranucleotide repeats, of which 20 were selected for 2 multiplexes based on amplifi cation success and band size. Eleven of these loci showed polymorphism in two populations of Khaya senegalensis and amplifi ed in individuals from across the species range. Conclusions: These new microsatellite loci will be useful for investigation of the evolutionary and conservation genetics of Khaya senegalensis

Details

Title: Development and characterization of microsatellite loci for Khaya senegalensis (Meliaceae)
Authors: G J Sexton (Author) - University of Queensland
Celine H Frere (Author) - University of Queensland
M J Dieters (Author) - University of Queensland
I D Godwin (Author) - University of Queensland
P J Prentis (Author) - University of Queensland
Publication details: American Journal of Botany, Vol.97(11), pp.e111-e113
Publisher: Botanical Society of America, Inc.
Date published: 2010
DOI: 10.3732/ajb.1000300
ISSN: 0002-9122
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99448939302621
Output Type: Journal article

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