Development and Interrogation of a Transcriptomic Resource for the Giant Triton Snail (Charonia tritonis)

Anne H Klein; Cherie A Motti; Adam Hillberg; Tomer Ventura; P Thomas-Hall; T Armstrong; T Barker; Paul Whatmore; Scott Cummins

doi:10.1007/s10126-021-10042-7

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Development and Interrogation of a Transcriptomic Resource for the Giant Triton Snail (Charonia tritonis)

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Development and Interrogation of a Transcriptomic Resource for the Giant Triton Snail (Charonia tritonis)

Anne H Klein, Cherie A Motti, Adam Hillberg, Tomer Ventura, P Thomas-Hall, T Armstrong, T Barker, Paul Whatmore and Scott Cummins

Marine Biotechnology, Vol.23(3), pp.501-515

2021

DOI: https://doi.org/10.1007/s10126-021-10042-7

PMCID: PMC8270824

PMID: 34191212

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Abstract

Olfaction

Cytochrome P450

G protein-coupled receptor

Neuropeptide

Crown-of-thorns starfish

Gastropod molluscs are among the most abundant species that inhabit coral reef ecosystems. Many are specialist predators, along with the giant triton snail Charonia tritonis (Linnaeus, 1758) whose diet consists of Acanthaster planci (crown-ofthorns starfsh), a corallivore known to consume enormous quantities of reef-building coral. C. tritonis are considered vulnerable due to overexploitation, and a decline in their populations is believed to have contributed to recurring A. planci population outbreaks. Aquaculture is considered one approach that could help restore natural populations of C. tritonis and mitigate coral loss; however, numerous questions remain unanswered regarding their life cycle, including the molecular factors that regulate their reproduction and development. In this study, we have established a reference C. tritonis transcriptome derived from developmental stages (embryo and veliger) and adult tissues. This was used to identify genes associated with cell signalling, such as neuropeptides and G protein-coupled receptors (GPCRs), involved in endocrine and olfactory signalling. A comparison of developmental stages showed that several neuropeptide precursors are exclusively expressed in post-hatch veligers and functional analysis found that FFamide stimulated a signifcant (20.3%) increase in larval heart rate. GPCRs unique to veligers, and a diversity of rhodopsin-like GPCRs located within adult cephalic tentacles, all represent candidate olfactory receptors. In addition, the cytochrome P450 superfamily, which participates in the biosynthesis and degradation of steroid hormones and lipids, was also found to be expanded with at least 91 genes annotated, mostly in gill tissue. These fndings further progress our understanding of C. tritonis with possible application in developing aquaculture methods.

Details

Title: Development and Interrogation of a Transcriptomic Resource for the Giant Triton Snail (Charonia tritonis)
Authors: Anne H Klein (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Cherie A Motti (Author) - Australian Institute of Marine Science
Adam Hillberg (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Tomer Ventura (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
P Thomas-Hall (Author) - Australian Institute of Marine Science
T Armstrong (Author) - Australian Institute of Marine Science
T Barker (Author) - Australian Institute of Marine Science
Paul Whatmore (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Scott Cummins (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Publication details: Marine Biotechnology, Vol.23(3), pp.501-515
Publisher: Springer New York LLC
Date published: 2021
DOI: 10.1007/s10126-021-10042-7
ISSN: 1436-2236
PMID: 34191212; PMC8270824
Copyright note: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Organisation Unit: University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99548405202621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology; Marine & Freshwater Biology