Journal article
Detection of novel chlamydiae in cats with ocular disease
American Journal of Veterinary Research, Vol.64(11), pp.1421-1428
2003
Abstract
Objective - To detect and characterize the full range of chlamydial infections in cats with ocular disease by use of polymerase chain reaction (PCR) assays, cytologic examination, immunohistochemical analysis, and evaluation of clinical information including status for feline herpesvirus-1 (FeHV-1). Sample Population - DNA extracted from 226 conjunctival samples obtained from cats with clinically diagnosed keratitis or conjunctivitis and 30 conjunctival samples from healthy cats. Procedure - PCR assays for the 16S rRNA gene specific for the order Chlamydiales and a new Chlamydophila felis (formerly Chlamydia psittaci) species-specific 23S rRNA gene were performed. Seventy-four conjunctival samples were prepared with Romanowsky-type stain, grouped on the basis of inflammatory pattern, and screened for chlamydial inclusions by use of immunohistochemical analysis. Clinical information and FeHV-1 status were recorded. Results - 26 (12%) specimens had positive results for the only known feline chlamydial pathogen, C felis. Surprisingly, an additional 88 (39%) were positive for non-C felis chlamydial DNA. Identification of non-C felis chlamydial DNA by direct sequencing revealed 16S rRNA gene sequences that were 99% homologous to the sequence for Neochlamydia hartmannellae, an amebic endosymbiont. Chlamydial prevalence was significantly higher in cats with ocular disease. Conclusions and Clinical Relevance - Application of a broad-range detection method resulted in identification of a new agent associated with ocular disease in cats. Finding chlamydia-like agents such as N hartmannellae in coinfections with their obligate amebic host, Hartmannella vermiformis, raises questions about the potential role of these microorganisms in causation or exacerbation of ocular disease in cats.
Details
- Title
- Detection of novel chlamydiae in cats with ocular disease
- Authors
- W von Bomhard (Author)Adam Polkinghorne (Author)Z Huat Lu (Author)L Vaughan (Author)A Vögtlin (Author)D R Zimmermann (Author)B Spiess (Author)A Pospischil (Author)
- Publication details
- American Journal of Veterinary Research, Vol.64(11), pp.1421-1428
- Publisher
- American Veterinary Medical Association
- Date published
- 2003
- DOI
- 10.2460/ajvr.2003.64.1421
- ISSN
- 0002-9645
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99448871402621
- Output Type
- Journal article
Metrics
2 File views/ downloads
809 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Veterinary Sciences
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites