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Detection of Leishmania (Mundinia) macropodum (Kinetoplastida: Trypanosomatidae) and heterologous Leishmania species antibodies among blood donors in a region of Australia with marsupial Leishmania endemicity
Journal article   Open access   Peer reviewed

Detection of Leishmania (Mundinia) macropodum (Kinetoplastida: Trypanosomatidae) and heterologous Leishmania species antibodies among blood donors in a region of Australia with marsupial Leishmania endemicity

Elina Panahi, Danielle I Stanisic, Eloise B Skinner, Helen Faddy, Megan K Young and Lara J Herrero
International Journal of Infectious Diseases, Vol.130, pp.42-47
2023
PMID: 36241162
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1-s2.0-S1201971222005458-main663.01 kBDownloadView
Published Version Open Access CC BY-NC-ND V4.0
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https://doi.org/10.1016/j.ijid.2022.10.006View
Published Version

Abstract

serology IgG subclasses Leishmania parasites human cutaneous leishmaniasis
Objectives: The Australian Leishmania (Mundinia) macropodum parasite causes cutaneous leishmaniasis (CL) among marsupial species. While CL is a major public health burden globally, it is not clear if humans are naturally exposed to the unique L. macropodum. To assess whether humans have an IgG response to L. macropodum, we examined anti-Leishmania antibodies among humans residing in a region of marsupial Leishmania endemicity in Australia. Methods: Employing a serological enzyme-linked immunosorbent assay, we characterized Leishmania-specific IgG and IgG subclass responses to soluble Leishmania antigen (SLA) from L. macropodum and other Leishmania species (L. donovani, L. major and L. mexicana) in 282 blood donor samples. Results: We found 20.57% of individuals demonstrated a positive total IgG response to L. macropodum. For individuals with antibodies to SLA from one Leishmania species, there was no increased likelihood of recognition to other Leishmania species. For samples with detectable L. macropodum IgG, IgG1 and IgG2 were the prevalent subclasses detected. Conclusions: It is not yet clear whether the IgG antibody detection in this study reflects exposure to Leishmania parasites or a cross-reactive immune response that was induced against an unrelated immunogen. Future studies should investigate whether L. macropodum can result in a viable infection in humans.

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