Detection of Chlamydia trachomatis in urine using enzyme immunoassay and DNA amplification

S J Rasmussen; H Smith-Vaughan; M Nelson; S W Chan; Peter Timms; A G Capon

doi:10.1006/mcpr.1993.1063

Back

Detection of Chlamydia trachomatis in urine using enzyme immunoassay and DNA amplification

Journal article

Peer reviewed

Detection of Chlamydia trachomatis in urine using enzyme immunoassay and DNA amplification

S J Rasmussen, H Smith-Vaughan, M Nelson, S W Chan, Peter Timms and A G Capon

Molecular and Cellular Probes, Vol.7(6), pp.425-430

1993

DOI: https://doi.org/10.1006/mcpr.1993.1063

Files and links (1)

url

https://doi.org/10.1006/mcpr.1993.1063View

Published Version

Abstract

PCR

Chlamydia trachomatis

urine

Chlamydiazyme

DNA

The suitability of urine specimens for the detection of Chlamydia trachomatis infections in men was assessed. Urethral swabs from 301 patients were cultured for C. trachomatis, and the results were compared with results obtained from Chlamydiazyme. The results of 298 specimens were also compared with results obtained from PCR analysis of first-void urine specimens. The sensitivity of confirmed Chlamydiazyme analysis was 93% and the specificity was greater than 99% compared with culture. The sensitivity of the PCR metod was 100% compared with culture. Chlamydia trachomatis was detected by PCR in an additional three specimens from which C. trachomatis could not be cultured. Urine appears to be an appropriate specimen for the detection of C. trachomatis antigens and nucleic acids.

Details

Title: Detection of Chlamydia trachomatis in urine using enzyme immunoassay and DNA amplification
Authors: S J Rasmussen (Author) - Queensland University of Technology
H Smith-Vaughan (Author) - Queensland University of Technology
M Nelson (Author) - Queensland University of Technology
S W Chan (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
A G Capon (Author) - Queensland University of Technology
Publication details: Molecular and Cellular Probes, Vol.7(6), pp.425-430
Publisher: Academic Press
Date published: 1993
DOI: 10.1006/mcpr.1993.1063
ISSN: 0890-8508
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449417602621
Output Type: Journal article

Metrics

552 Record Views

8 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Cell Biology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites