Journal article
Detection and characterization of verotoxin-producing strains among sorbitol non-fermenting Escherichia coli
Eastern Mediterranean Health Journal, Vol.7(4/5), pp.756-762
2001
Abstract
The presence of genes for verotoxin 1 and 2 (VT1 and 2) among sorbitol non-fermenting Escherichia coli isolates from diarrhoeal cases was assessed using polymerase chain reaction assay. Of 60 (88%) positive isolates, 37 (62%) harboured VT1 and 23 (38%) both VT1 and VT2. In HeLa cell adherence assay, 48 (71%) isolates exhibited mannose-resistant adherence to HeLa cells. Multidrug resistance was observed in 56 (82%) isolates, with ampicillin, chloramphenicol, streptomycin, sulfamethoxazole-trimethoprim and tetracycline pattern being the most common. There were 13 common and 22 single biochemical phenotypes identified. Isolates belonging to common biochemical phenotypes normally had a similar pattern of adherence and VT production, but differed greatly in their pattern of antibiotic resistance, pointing to a high rate of antibiotic-resistance transfer among these isolates.
Details
- Title
- Detection and characterization of verotoxin-producing strains among sorbitol non-fermenting Escherichia coli
- Authors
- A Jafari (Author) - Pasteur Institute of Iran, IranMohammad Katouli (Author) - Pasteur Institute of Iran, IranF Shokouhi (Author) - Pasteur Institute of Iran, IranS Bouzari (Author) - Pasteur Institute of Iran, Iran
- Publication details
- Eastern Mediterranean Health Journal, Vol.7(4/5), pp.756-762
- Publisher
- World Health Organization, Regional Office for the Eastern Mediterranean Egypt
- Date published
- 2001
- DOI
- 10.26719/2001.7.4-5.756
- ISSN
- 1020-3397
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450492202621
- Output Type
- Journal article
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