Journal article
Detecting co-cultivation induced chemical diversity via 2D NMR fingerprints
Microbiology Australia, Vol.40(4), pp.186-198
2019
Abstract
Rediscovery of already known compounds is a major issue in microbial natural product drug discovery. In recent years, progress has been made in developing more efficient analytical approaches that quickly identify known compounds in a sample to minimise rediscovery. In parallel, whole genome sequencing of microorganisms has revealed their immense potential to produce secondary metabolites, yet the majority of biosynthetic genes remain silent under common laboratory culturing conditions. Therefore, increased research has focused on optimising culturing methods to activate the silent biosynthetic gene clusters. Co-cultivation of different microbial strains can activate biosynthetic gene clusters that remain silent under standard laboratory fermentations involving mono-cultures, hence, the technique has great potential for natural product drug discovery. However, innovative methods are still needed to evaluate the success of any co-cultured fermentation end-product. Here, the application of HSQC-TOCSY NMR spectra and subsequent PCoA to identify changes in the metabolite diversity induced through co-cultivation is described.
Details
- Title
- Detecting co-cultivation induced chemical diversity via 2D NMR fingerprints
- Authors
- Larissa Buedenbender (Author) - Griffith UniversityAnthony R Carroll (Author) - Griffith UniversityD Ipek Kurtboke (Author) - University of the Sunshine Coast - School of Science & Engineering
- Publication details
- Microbiology Australia, Vol.40(4), pp.186-198
- Publisher
- C S I R O Publishing
- Date published
- 2019
- DOI
- 10.1071/MA19054
- ISSN
- 1324-4272; 1324-4272
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451392802621
- Output Type
- Journal article
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- Domestic collaboration
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- Microbiology
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