Detecting arthropod intraguild predation in the field

Alexandre Aebi; Peter M. J. Brown; Patrick De Clercq; Louis Hautier; Andy Howe; Brecht Ingels; Hans-Peter Ravn; John J. Sloggett; Renate Zindel; Alison Thomas

doi:10.1007/s10526-011-9378-2

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Detecting arthropod intraguild predation in the field

Journal article

Peer reviewed

Detecting arthropod intraguild predation in the field

Alexandre Aebi, Peter M. J. Brown, Patrick De Clercq, Louis Hautier, Andy Howe, Brecht Ingels, Hans-Peter Ravn, John J. Sloggett, Renate Zindel and Alison Thomas

BioControl, Vol.56(4), pp.429-440

2011

DOI: https://doi.org/10.1007/s10526-011-9378-2

Abstract

biological control

intra-guild predation

gut-content

barcoding

GC-MS

ELISA

environmental risk assessment

The process of biological control carries a distinct risk that an alien biological control agent (BCA) will become established as an invasive alien species with an associated threat to the local ecosystem biodiversity. It is imperative that a wide-ranging environmental risk assessment (ERA) is performed before the release of any BCA. This should include considering various potential but difficult to observe ecological interactions between the BCA and members of the native community, including disruption of intraguild relationships. Detection of intraguild predation (IGP) events involving predatory arthropods in the field can be done by analyzing their gut contents. Polymerase chain reaction (PCR) is a sensitive and specific tool to identify target prey DNA within a predator's gut. This paper reviews the efficiency of a DNA based approach for detecting IGP in the field, compared with detection by the use of monoclonal antibodies or gas chromatography. Prey specificity, detection times after prey consumption, capacity for quantification, multiple prey targeting and the time and costs involved in developing and using the different methods are considered.

Details

Title: Detecting arthropod intraguild predation in the field
Authors: Alexandre Aebi (Corresponding Author) - Agroscope
Peter M. J. Brown (Author) - Anglia Ruskin University
Patrick De Clercq (Author) - Ghent University
Louis Hautier (Author) - Walloon Agricultural Research Centre
Andy Howe (Author) - University of Copenhagen
Brecht Ingels (Author) - Ghent University
Hans-Peter Ravn (Author) - University of Copenhagen
John J. Sloggett (Author)
Renate Zindel (Author) - Agroscope
Alison Thomas (Author) - Anglia Ruskin University
Publication details: BioControl, Vol.56(4), pp.429-440
Publisher: Springer Dordrecht
Date published: 2011
DOI: 10.1007/s10526-011-9378-2
ISSN: 1573-8248
Organisation Unit: Forest Research Institute; Forest Industries Research Centre
Language: English
Record Identifier: 99980280902621
Output Type: Journal article