Destabilisation of Argonaute 2 generates a truncated protein: halfAgo2

Sarah Bajan; Michael Johnston; Gyorgy Hutvagner

doi:10.19185/matters.201811000001

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Destabilisation of Argonaute 2 generates a truncated protein: halfAgo2

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Destabilisation of Argonaute 2 generates a truncated protein: halfAgo2

Sarah Bajan, Michael Johnston and Gyorgy Hutvagner

Matters, pp.1-8

2018

DOI: https://doi.org/10.19185/matters.201811000001

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Abstract

Argonaute proteins

MicroRNA

RISC

Argonaute 2

Argonaute 2 Stability

The Argonaute 2 (Ago2) protein is an essential effector protein in miRNA-mediated mechanisms that regulate gene expression. Ago2 directly binds to the miRNA, forming the RISC. RISC function is critical to controlling key biological processes and when dysregulated can result in disease pathogenesis. Understanding Ago2 protein stability and turnover will further our understanding in how RISC function is regulated. In human cells, we discovered a previously unidentified ~55 kDa protein that is a truncated form of Ago2, that is formed from proteolytic cleavage of the full length Ago2 protein. Further experiments are needed to determine (i) the detailed mechanism that forms halfAgo2 (ii) the cellular or environmental triggers or stresses that initiate halfAgo2 production and (iii) if halfAgo2 has a potentially new role in gene regulation.

Details

Title: Destabilisation of Argonaute 2 generates a truncated protein: halfAgo2
Authors: Sarah Bajan (Author) - University of Technology Sydney
Michael Johnston (Author) - University of Technology Sydney
Gyorgy Hutvagner (Author) - University of Technology Sydney
Publication details: Matters, pp.1-8
Publisher: ScienceMatters AG
Date published: 2018
DOI: 10.19185/matters.201811000001
ISSN: 2297-8240
Copyright note: Distributed under the terms of a Creative Commons Attribution 4.0 International License.
Grants: Dissecting key steps of the miRNA-mediated gene regulation and its implication in immune response and cancer, FT110100455, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Health - Biomedicine; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99472506602621
Output Type: Journal article

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