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Description of a new species of sucking louse Hoplopleura kuhnludwigi (Phthiraptera: Anoplura: Hoplopleuridae) from the Cape York rat Rattus leucopus cooktownensis (Rodentia: Muridae) in Australia using conventional methodology and novel epiflourescence microscopy
Journal article   Open access   Peer reviewed

Description of a new species of sucking louse Hoplopleura kuhnludwigi (Phthiraptera: Anoplura: Hoplopleuridae) from the Cape York rat Rattus leucopus cooktownensis (Rodentia: Muridae) in Australia using conventional methodology and novel epiflourescence microscopy

Constantin Constantinoiu, Wei Wang, Cadhla Firth, Richard Duffy, Jaqueline Picard and Bruce Gummow
Parasitology International, Vol.106, pp.1-18
2025
PMID: 39608566
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1-s2.0-S1383576924001533-main18.45 MBDownloadView
Published Version Open Access CC BY V4.0
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https://doi.org/10.1016/j.parint.2024.103002View
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Abstract

sucking lice Hoplopleura kuhnludwigi Rattus leucopus cooktownensis Autofluorescence new species epiflurescence microscopy
A new species of sucking louse, Hoplopleura kuhnludwigi (Phthiraptera: Hoplopleuridae), from the Cape York rat Rattus leucopus cooktownensis Tate, 1951 (Rodentia: Muridae) is described and illustrated with photos and drawings. The host rat(s) were trapped in Northeast Queensland, Australia and identified using morphological characters and DNA sequencing. Hoplopleura kuhnludwigi is the first louse species to be described on R. leucopus. The morphology of the new louse species is contrasted with the morphology of Hoplopleura spp. previously described in Australian murids and shown to be different from other louse species that infest Australian native murids. Both conventional lice processing and staining methodology and a novel method, based on auto- fluorescence of chitin, have been used to describe and illustrate the diagnostic characters of H. kuhnludwigi. Processing of H. kuhnludwigi for epifluorescence microscopy (EM) is fast and easy, and the examination of the lice processed by this method can improve the accuracy of the description and identification of the lice species from genus Hoplopleura and potentially other louse species.

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