Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn

W F Smyth; T J Smyth; V N Ramachandran; F O'Donnell; Peter R Brooks

doi:10.1016/j.trac.2011.09.015

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Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn

Journal article

Peer reviewed

Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn

W F Smyth, T J Smyth, V N Ramachandran, F O'Donnell and Peter R Brooks

Trends in Analytical Chemistry, Vol.33, pp.46-54

2012

DOI: https://doi.org/10.1016/j.trac.2011.09.015

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Abstract

antibacterial activity

column chromatography

dereplication

electrospray ionization-ion trap mass spectrometry (ESI-MSn)

Ficus coronate

liquid chromatography-ESI-MS (LC-ESI-MS)

Melicope vitiflora

phytochemical

Ruta graveolens

thin layer chromatography

This review discusses how crude extracts of selected plants are subjected to column chromatography and the resulting fractions tested for their antibacterial activity towards organisms (e.g., methicillin-resistant-Staphylococcus aureus). We discuss the bioactive column-chromatography fractions being further separated by preparative thin-layer chromatography and the resulting bands being investigated by high-performance liquid chromatography-electrospray ionization-ion trap mass spectrometry (LC-ESI-MS) and ESI-MSn. We then show how the resulting retention times, molecular masses, their fragmentation patterns, the Chemnet database and an in-house quinoline database are used in the dereplication process by structural elucidation of some of the compounds when compared with known structures of natural origin. Some molecular masses and the corresponding fragmentations do not correlate with any known compounds, thus revealing potentially novel natural products that could be investigated on a larger scale and could ultimately find application as new drugs against MRSA and other multi-drug-resistant microorganisms.

Details

Title: Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn
Authors: W F Smyth (Author) - University of Ulster, United Kingdom
T J Smyth (Author) - University of Ulster, United Kingdom
V N Ramachandran (Author) - University of Ulster, United Kingdom
F O'Donnell (Author) - University of Ulster, United Kingdom
Peter R Brooks (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Trends in Analytical Chemistry, Vol.33, pp.46-54
Publisher: Elsevier BV
Date published: 2012
DOI: 10.1016/j.trac.2011.09.015
ISSN: 0165-9936
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449871802621
Output Type: Journal article

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