Degradation of aliphatic halogen-substituted pesticides by dehalogenase isolated from Pseudomonas alcaligenes. Identification and properties of the enzyme

M D Busto; P P Smith; M Perez-Mateos; Richard G Burns

doi:10.1016/0048-9697(92)90152-I

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Degradation of aliphatic halogen-substituted pesticides by dehalogenase isolated from Pseudomonas alcaligenes. Identification and properties of the enzyme

Journal article

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Degradation of aliphatic halogen-substituted pesticides by dehalogenase isolated from Pseudomonas alcaligenes. Identification and properties of the enzyme

M D Busto, P P Smith, M Perez-Mateos and Richard G Burns

Science of the Total Environment, Vol.123-124(C), pp.267-277

1992

DOI: https://doi.org/10.1016/0048-9697(92)90152-I

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Abstract

2,2-dichloropropionate

pseudomonas alcaligenes

dalapon

monochloroacetic acid

dichloroacetic acid

2-monochloropropionic acid

2,2-dichloropropionic acid

Some characteristics of a 2,2-dichloropropionate dehalogenase induced in a bacterial strain capable of degrading high concentrations of the herbicide dalapon were studied. Polyacrilamide gel electrophoresis of the crude cell free extracts identified only one type of dehalogenase. The single enzymatic protein showed activity against a variety of chlorinated aliphatic acids but differed in their activity levels. Thus activity in ?mol substrate converted (mg protein)-1 min-1 was 2-monochloropropionate 0.65, 2,2-dichloropropionate 0.56, 2-monochloroacetate 1.70 and 2,2-dichloroacetate 1.00. In the crude extracts, the enzyme activity against 2,2-dichloropropionate was optimal at a broad pH range with a mid-point at pH 9.5 and apparent Km values were within the range 0.23-0.73 mM. © 1992.

Details

Title: Degradation of aliphatic halogen-substituted pesticides by dehalogenase isolated from Pseudomonas alcaligenes. Identification and properties of the enzyme
Authors: M D Busto (Author) - University of Valladolid, Spain
P P Smith (Author) - University of Kent, United Kingdom
M Perez-Mateos (Author) - University of Valladolid, Spain
Richard G Burns (Author) - University of Kent, United Kingdom
Publication details: Science of the Total Environment, Vol.123-124(C), pp.267-277
Publisher: Elsevier BV
Date published: 1992
DOI: 10.1016/0048-9697(92)90152-I
ISSN: 0048-9697
Organisation Unit: University of the Sunshine Coast, Queensland; Office of the Deputy Vice-Chancellor (Research and Innovation)
Language: English
Record Identifier: 99449826102621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Environmental Sciences