Defining the E-Cadherin Repressor Interactome in Epithelial-Mesenchymal Transition: The PMC42 Model as a Case Study

H J Hugo; M I Kokkinos; T Blick; M L Ackland; E W Thompson; D F Newgreen

doi:10.1159/000320174

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Defining the E-Cadherin Repressor Interactome in Epithelial-Mesenchymal Transition: The PMC42 Model as a Case Study

Journal article

Peer reviewed

Defining the E-Cadherin Repressor Interactome in Epithelial-Mesenchymal Transition: The PMC42 Model as a Case Study

H J Hugo, M I Kokkinos, T Blick, M L Ackland, E W Thompson and D F Newgreen

Cells, Tissues, Organs, Vol.193, pp.23-40

2011

DOI: https://doi.org/10.1159/000320174

PMID: 21051859

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Abstract

breast cancer

epithelial-mesenchymal transition

E-cadherin transcriptional repression

Inter-regulation

Epithelial-mesenchymal transition (EMT) is a feature of migratory cellular processes in all stages of life, including embryonic development and wound healing. Importantly, EMT features cluster with disease states such as chronic fibrosis and cancer. The dissolution of the E-cadherin-mediated adherens junction (AJ) is a key preliminary step in EMT and may occur early or late in the growing epithelial tumour. This is a first step for tumour cells towards stromal invasion, intravasation, extravasation and distant metastasis. The AJ may be inactivated in EMT by directed E-cadherin cleavage; however, it is increasingly evident that the majority of AJ changes are transcriptional and mediated by an expanding group of transcription factors acting directly or indirectly to repress E-cadherin expression. A review of the current literature has revealed that these factors may regulate each other in a hierarchical pattern where Snail1 (formerly Snail) and Snail2 (formerly Slug) are initially induced, leading to the activation of Zeb family members, TCF3, TCF4, Twist, Goosecoid and FOXC2. Within this general pathway, many inter-regulatory relationships have been defined which may be important in maintaining the EMT phenotype. This may be important given the short half-life of Snail1 protein. We have investigated these inter-regulatory relationships in the mesenchymal breast carcinoma cell line PMC42 (also known as PMC42ET) and its epithelial derivative, PMC42LA. This review also discusses several newly described regulators of E-cadherin repressors including oestrogen receptor-α and new discoveries in hypoxia- and growth factor-induced EMT. Finally, we evaluated how these findings may influence approaches to current cancer treatment.

Details

Title: Defining the E-Cadherin Repressor Interactome in Epithelial-Mesenchymal Transition: The PMC42 Model as a Case Study
Authors: H J Hugo (Author) - St Vincents Institute of Medical Research
M I Kokkinos (Author) - University of Melbourne
T Blick (Author) - St Vincents Institute of Medical Research
M L Ackland (Author) - Deakin University
E W Thompson (Author) - St Vincents Institute of Medical Research
D F Newgreen (Author) - Murdoch Childrens Research Institute
Publication details: Cells, Tissues, Organs, Vol.193, pp.23-40
Publisher: S. Karger AG
DOI: 10.1159/000320174
ISSN: 1422-6421
PMID: 21051859
Organisation Unit: School of Health and Sport Sciences - Legacy; University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99518707602621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Anatomy & Morphology; Cell Biology; Developmental Biology

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