Decreased efferocytosis and mannose binding lectin in the airway in bronchiolitis obliterans syndrome

Sandra Hodge; Melinda M Dean; Greg L Hodge; M Holmes; P N Reynolds

doi:10.1016/j.healun.2011.01.710

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Decreased efferocytosis and mannose binding lectin in the airway in bronchiolitis obliterans syndrome

Journal article

Open access

Peer reviewed

Decreased efferocytosis and mannose binding lectin in the airway in bronchiolitis obliterans syndrome

Sandra Hodge, Melinda M Dean, Greg L Hodge, M Holmes and P N Reynolds

Journal of Heart and Lung Transplantation, Vol.30(5), pp.589-595

2011

DOI: https://doi.org/10.1016/j.healun.2011.01.710

PMID: 21481686

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Abstract

lung transplant

BOS

efferocytosis

macrophage

complement

BAL

mannose binding lectin

Background: Mannose binding lectin (MBL) is a key mediator of both innate immunity and efferocytosis (phagocytosis of apoptotic cells) in the airway. Defective efferocytosis results in a net increase in apoptotic material that can undergo secondary necrosis, leading to tissue damage and chronic inflammation. We have shown reduced MBL and efferocytosis in other chronic inflammatory lung diseases; we therefore hypothesized that reduced MBL and efferocytosis in the airways may be a determinant of bronchiolitis obliterans syndrome (BOS) after lung transplantation. Methods: We investigated MBL (enzyme-linked immunosorbent assay [ELISA]), MBL-mediated complement deposition (UC4, ELISA), and efferocytosis of apoptotic bronchial epithelial cells (flow cytometry) in bronchoalveolar lavage (BAL) and peripheral blood from 75 lung transplant recipients, comprising 16 with stable graft function, 34 stable with proven infection, 25 with BOS, and 14 healthy controls. Results: In plasma, MBL levels were highly variable (0–17.538 μg/ml), but increased in infected patients vs control (p = 0.09) or stable groups (p = 0.003). There was a similar increase in UC4 in infected patients and a significant correlation between MBL and UC4. There was no correlation between MBL and time after transplant. In BAL, MBL levels were less variable (0–73.3 ng/ml) and significantly reduced in patients with BOS vs controls and stable groups. Efferocytosis was significantly reduced in the BOS group vs control and stable groups (mean [SEM] control, 20% [1.3%]; stable, 20.5% [2.5%]; infected, 17.3% [2.8%]; BOS, 11.3% [1.5%], p = 0.04). Conclusions: Low levels of MBL in the airway may play a role in reduced efferocytosis, subsequent tissue damage, and BOS after lung transplantation.

Details

Title: Decreased efferocytosis and mannose binding lectin in the airway in bronchiolitis obliterans syndrome
Authors: Sandra Hodge (Author) - Royal Adelaide Hospital
Melinda M Dean (Author) - Australian Red Cross Blood Service
Greg L Hodge (Author) - Royal Adelaide Hospital
M Holmes (Author) - Royal Adelaide Hospital
P N Reynolds (Author) - Royal Adelaide Hospital
Publication details: Journal of Heart and Lung Transplantation, Vol.30(5), pp.589-595
Publisher: Elsevier Inc.
Date published: 2011
DOI: 10.1016/j.healun.2011.01.710
ISSN: 1053-2498; 1557-3117; 1053-2498
PMID: 21481686
Organisation Unit: School of Health - Biomedicine; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99513782902621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Cardiac & Cardiovascular Systems; Respiratory System; Surgery; Transplantation

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