Journal article
Decoctions from Citrus reticulata Blanco seeds protect the uroepithelium against Escherichia coli invasion
Journal of Ethnopharmacology, Vol.150(2), pp.770-774
2013
Abstract
Ethnopharmacological relevance: Traditional usage suggests Citrus reticulata Blanco seeds have beneficial effects against infection. The purpose of this study was to investigate the effect of C. reticulata on the uroepithelium and to determine the mechanisms responsible for protection against urinary tract infection (UTI). Materials and methods: Human bladder cell lines T24 and 5637 were employed in a cell culture infection model to determine the effects of C. reticulata treatment on Escherichia coli adherence and invasion of the uroepithelium. β1 integrin and caveolin-1 mRNA expression was assessed using RT real-time PCR. β1 integrin protein expression was confirmed by Western Blot. The effect of C. reticulata on bacteria was investigated using antibacterial sensitivity, yeast agglutination and biofilm assays. Results: C. reticulata treatment decreased β1 integrin expression and reduced bacterial invasion while adhesion of uroepithelial cells was not affected. Caveolin-1 expression was not influenced either and C. reticulata did neither exhibit any direct antimicrobial effect nor interfered with type 1 fimbriae binding. Conclusions: Our results show that C. reticulata has a protective effect on the uroepithelium as seen by reduced bacterial invasion of uroepithelial cells. These properties suggest that seeds from C. reticulata may have therapeutic potential in preventing UTI.
Details
- Title
- Decoctions from Citrus reticulata Blanco seeds protect the uroepithelium against Escherichia coli invasion
- Authors
- Tara L Vollmerhausen (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringNubia Ramos (Author) - Karolinska Institute, SwedenDang Thi Ngoc Dzung (Author) - Hanoi Medical University, VietnamAnnelie Brauner (Author) - Karolinska Institute, Sweden
- Publication details
- Journal of Ethnopharmacology, Vol.150(2), pp.770-774
- Publisher
- Elsevier Ireland Ltd.
- Date published
- 2013
- DOI
- 10.1016/j.jep.2013.09.050
- ISSN
- 0378-8741
- Copyright note
- Copyright © 2013. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy
- Language
- English
- Record Identifier
- 99448997302621
- Output Type
- Journal article
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- Chemistry, Medicinal
- Integrative & Complementary Medicine
- Pharmacology & Pharmacy
- Plant Sciences
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