De-novo transcriptome analysis of the banana shrimp (Fenneropenaeus merguiensis) and identification of genes associated with reproduction and development

Daniel Powell; Wayne R Knibb; Courtney Remilton; Abigail Elizur

doi:10.1016/j.margen.2015.04.006

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De-novo transcriptome analysis of the banana shrimp (Fenneropenaeus merguiensis) and identification of genes associated with reproduction and development

Journal article

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De-novo transcriptome analysis of the banana shrimp (Fenneropenaeus merguiensis) and identification of genes associated with reproduction and development

Daniel Powell, Wayne R Knibb, Courtney Remilton and Abigail Elizur

Marine Genomics, Vol.22, pp.71-78

2015

DOI: https://doi.org/10.1016/j.margen.2015.04.006

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Abstract

de-novo transcriptome

fenneropenaeus merguiensis

crustacean

shrimp

The banana shrimp Fenneropenaeus merguiensis is a commercially important marine crustacean for world aquaculture and fisheries. Despite this, limited genetic information is available for it and many other penaeid shrimp species. Here we present the first in-depth analysis of the transcriptional content of 8 different tissues from the banana shrimp using RNA-Seq technologies. A total of over 1 million single-end and over 49 million paired-end reads were obtained from Roche 454FLX and illumina sequencing platforms, respectively, resulting in an assembly of 124,631 transcripts with an N50 of 1,332 and mean length of 514 nt. A total of 59,179 putative protein sequences obtained from the assembled transcripts were annotated using public protein sequence databases and assigned 20,430 BLAST hits, 16,866 GO terms and 13,304 KOG categories. Further analysis revealed a rich set of transcript sequences exhibiting homology with genes associated with reproduction, sex determination and development and distinguished the tissues responsible for this expression. This report adds a substantial contribution to the sequence data currently available for F. merguiensis, providing valuable resources for further research.

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Title: De-novo transcriptome analysis of the banana shrimp (Fenneropenaeus merguiensis) and identification of genes associated with reproduction and development
Authors: Daniel Powell (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Wayne R Knibb (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Courtney Remilton (Author) - Seafarms Pty Ltd
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Marine Genomics, Vol.22, pp.71-78
Publisher: Elsevier BV
Date published: 2015
DOI: 10.1016/j.margen.2015.04.006
ISSN: 1874-7787
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; Office of Research; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449302902621
Output Type: Journal article

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