Journal article
De novo assembly of the transcriptome of Acanthaster planci testes
Molecular Ecology Resources, Vol.15(4), pp.953-966
2015
Abstract
A key strategy to reduce coral loss is the development of effective control method for the corallivorous crown-of-thorns sea star (Acanthaster planci), an omnipresent scourge and threat to the biodiversity of reefs in the Indo-Pacific region. Limited genetic resources are available for this highly fecund species. In the present study, we explored one aspect at the heart of A. planci outbreaks, the male reproductive system. Using high-throughput sequencing technology, we report for first time the production of a comprehensive transcriptomic dataset for the testes of A. placni that can aid in understanding the molecular mechanisms involved in A. planci spermatogenesis and fertilisation. Through de novo transcriptome sequencing, we produced 52,965,998 raw reads corresponding to 4.76 Gb clean read data. From this, 243,870 contigs were assembled with Trinity and used to construct 92,792 unigenes. Distinct genes were then annotated with Blastx yielding 30,810 unigenes above the cut-off e-value set at 10-5, with ESTscan database query analyses yielding up to 5,366 unigenes to known hits. The identification of genes directly involved in sperm development (DEAD-box family proteins), motility, fertilisation and signalling (Bindin/Speract receptor) are also discussed.
Details
- Title
- De novo assembly of the transcriptome of Acanthaster planci testes
- Authors
- Michael J Stewart (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringPraphaporn Stewart (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringJairo Rivera-Posada (Author) - James Cook University
- Publication details
- Molecular Ecology Resources, Vol.15(4), pp.953-966
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2015
- DOI
- 10.1111/1755-0998.12360
- ISSN
- 1755-098X
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449930902621
- Output Type
- Journal article
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- Biochemistry & Molecular Biology
- Ecology
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