DRB1 and DRB2 Are Required for an Appropriate miRNA-Mediated Molecular Response to Salt Stress in Arabidopsis thaliana

Joseph L. Pegler; Jackson M. J. Oultram; Christopher P. L. Grof; Andrew L. Eamens

doi:10.3390/plants14060924

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DRB1 and DRB2 Are Required for an Appropriate miRNA-Mediated Molecular Response to Salt Stress in Arabidopsis thaliana

Journal article

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Peer reviewed

DRB1 and DRB2 Are Required for an Appropriate miRNA-Mediated Molecular Response to Salt Stress in Arabidopsis thaliana

Joseph L. Pegler, Jackson M. J. Oultram, Christopher P. L. Grof and Andrew L. Eamens

Plants, Vol.14(6), pp.1-27

2025

DOI: https://doi.org/10.3390/plants14060924

PMCID: PMC11944917

PMID: 40265861

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Abstract

Arabidopsis thaliana (Arabidopsis)

salt stress

microRNA

miRNA

gene expression regulation

Double-stranded RNA binding (DRB) protein

DRB1

DRB2

In plants, microRNAs (miRNAs) and their target genes have been demonstrated to form an essential component of the molecular response to salt stress. In Arabidopsis thaliana (Arabidopsis), DOUBLE-STRANDED RNA BINDING1 (DRB1) and DRB2 are required to produce specific miRNA populations throughout normal development and in response to abiotic stress. The phenotypic and physiological assessment of 15-day-old wild-type Arabidopsis seedlings, and of the drb1 and drb2 mutants following a 7-day period of salt stress, revealed the drb2 mutant to be more sensitive to salt stress than the drb1 mutant. However, the assessment of miRNA abundance and miRNA target gene expression showed that the ability of both drb mutants to mount an appropriate miRNA-mediated molecular response to salt stress is defective. Furthermore, molecular profiling also showed that DRB1 and DRB2 are both required for miRNA production during salt stress, and that both a target transcript cleavage mode and a translational repression mode of RNA silencing are required to appropriately regulate miRNA target gene expression as part of the molecular response of Arabidopsis to salt stress. Taken together, the phenotypic, physiological, and molecular analyses performed here clearly show that all components of the miRNA pathway must be fully functional for Arabidopsis to mount an appropriate miRNA-mediated molecular response to salt stress.

Details

Title: DRB1 and DRB2 Are Required for an Appropriate miRNA-Mediated Molecular Response to Salt Stress in Arabidopsis thaliana
Authors: Joseph L. Pegler - University of Newcastle Australia
Jackson M. J. Oultram - University of Newcastle Australia
Christopher P. L. Grof - University of Newcastle Australia
Andrew L. Eamens (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Health - Biomedicine
Publication details: Plants, Vol.14(6), pp.1-27
Publisher: MDPI AG
Date published: 2025
DOI: 10.3390/plants14060924
ISSN: 2223-7747
PMID: 40265861; PMC11944917
Copyright note: © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Data Availability: All data reported here are available from the authors upon request.
Organisation Unit: School of Health - Biomedicine
Language: English
Record Identifier: 991113850202621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Plant Sciences

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