DNA amplification fingerprinting analysis of genetic variation within Fusarium oxysporum f.sp. zingiberi

Linda Pappalardo; Michael K Smith; S D Hamill; A M Stirling; David B McKay

doi:10.1071/AP08076

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DNA amplification fingerprinting analysis of genetic variation within Fusarium oxysporum f.sp. zingiberi

Journal article

Peer reviewed

DNA amplification fingerprinting analysis of genetic variation within Fusarium oxysporum f.sp. zingiberi

Linda Pappalardo, Michael K Smith, S D Hamill, A M Stirling and David B McKay

Australasian Plant Pathology, Vol.38(1), pp.51-54

2009

DOI: https://doi.org/10.1071/AP08076

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Abstract

Fusarium yellows

ginger

rhizome rot

Zingiber officinale

Genetic variation among 29 isolates of Fusarium oxysporum f.sp. zingiberi (Foz) collected from diseased ginger rhizome in production regions throughout Queensland was analysed using DNA amplification fingerprinting (DAF). Eight isolates of other Fusarium species and/or formae speciales were included for comparative analysis. Within the Foz isolates, three haplotypes were identified based on 17 polymorphic bands generated with five primers. Two groups showed very little genetic variation (98.6% similarity), whereas the third single isolate was quite distinct in terms of its molecular profile (77.2% similarity). Genetic similarity among the Fusarium solani, F. oxysporum f.sp. lycopersici and F. oxysporum f.sp. cubense races 1, 3 and 4 isolates compared well with the published literature.

Details

Title: DNA amplification fingerprinting analysis of genetic variation within Fusarium oxysporum f.sp. zingiberi
Authors: Linda Pappalardo (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Michael K Smith (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
S D Hamill (Author) - Queensland Department of Primary Industries and Fisheries
A M Stirling (Author) - Biological Crop Protection
David B McKay (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Publication details: Australasian Plant Pathology, Vol.38(1), pp.51-54
Publisher: C S I R O Publishing
DOI: 10.1071/AP08076
ISSN: 0815-3191
Organisation Unit: School of Science, Technology and Engineering; University of the Sunshine Coast, Queensland; School of Science and Engineering - Legacy
Language: English
Record Identifier: 99449658202621
Output Type: Journal article

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