DNA Heat Treatment for Improving qPCR Analysis of Human Adenovirus in Wastewater

Emily Rames; Anne H Roiko; Helen Stratton; Joanne Macdonald

doi:10.1007/s12560-017-9294-4

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DNA Heat Treatment for Improving qPCR Analysis of Human Adenovirus in Wastewater

Journal article

Open access

Peer reviewed

DNA Heat Treatment for Improving qPCR Analysis of Human Adenovirus in Wastewater

Emily Rames, Anne H Roiko, Helen Stratton and Joanne Macdonald

Food and Environmental Virology, Vol.9(3), pp.354-357

2017

DOI: https://doi.org/10.1007/s12560-017-9294-4

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Abstract

adenovirus

QPCR

inhibitors

health risk

wastewater

DNA heat treatment

PCR inhibitory substances in complex sample matrices can cause false negatives or under-estimation of target concentration. This study assessed DNA heat treatment for reducing inhibition during qPCR analysis of human adenovirus (HAdV) in wastewater samples. Inhibition was reduced by heat treating DNA, where mean HAdV concentration was increased by 0.71 log10 GC/L (and up to 3.04 log10 GC/L in one case), and replicate variability and false negatives were reduced. DNA heat treatment should be further investigated for improving reliability of HAdV concentration estimates in water, which can support more accurate assessment of health risks associated with viral pathogen exposure.

Details

Title: DNA Heat Treatment for Improving qPCR Analysis of Human Adenovirus in Wastewater
Authors: Emily Rames (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Anne H Roiko (Author) - Griffith University
Helen Stratton (Author) - Griffith University
Joanne Macdonald (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Food and Environmental Virology, Vol.9(3), pp.354-357
Publisher: Springer New York LLC
Date published: 2017
DOI: 10.1007/s12560-017-9294-4
ISSN: 1867-0334
Copyright note: Copyright The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation; Sustainability Research Cluster
Language: English
Record Identifier: 99450925602621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Environmental Sciences; Food Science & Technology; Microbiology; Virology

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