Journal article
DNA Heat Treatment for Improving qPCR Analysis of Human Adenovirus in Wastewater
Food and Environmental Virology, Vol.9(3), pp.354-357
2017
Abstract
PCR inhibitory substances in complex sample matrices can cause false negatives or under-estimation of target concentration. This study assessed DNA heat treatment for reducing inhibition during qPCR analysis of human adenovirus (HAdV) in wastewater samples. Inhibition was reduced by heat treating DNA, where mean HAdV concentration was increased by 0.71 log10 GC/L (and up to 3.04 log10 GC/L in one case), and replicate variability and false negatives were reduced. DNA heat treatment should be further investigated for improving reliability of HAdV concentration estimates in water, which can support more accurate assessment of health risks associated with viral pathogen exposure.
Details
- Title
- DNA Heat Treatment for Improving qPCR Analysis of Human Adenovirus in Wastewater
- Authors
- Emily Rames (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAnne H Roiko (Author) - Griffith UniversityHelen Stratton (Author) - Griffith UniversityJoanne Macdonald (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Food and Environmental Virology, Vol.9(3), pp.354-357
- Publisher
- Springer New York LLC
- Date published
- 2017
- DOI
- 10.1007/s12560-017-9294-4
- ISSN
- 1867-0334; 1867-0334
- Copyright note
- Copyright The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation; Sustainability Research Cluster
- Language
- English
- Record Identifier
- 99450925602621
- Output Type
- Journal article
- Research Statement
- false
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Environmental Sciences
- Food Science & Technology
- Microbiology
- Virology
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