DEFECTIVE EMBRYO AND MERISTEMS1 (DEM1) Is Essential for Cell Proliferation and Cell Differentiation in Tomato

Louisa Matthew; Melquiades E. C. Reyes; Christopher W. G. Mann; Alasdair W. McDowall; Andrew L. Eamens; Bernard J. Carroll

doi:10.3390/plants11192545

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DEFECTIVE EMBRYO AND MERISTEMS1 (DEM1) Is Essential for Cell Proliferation and Cell Differentiation in Tomato

Journal article

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DEFECTIVE EMBRYO AND MERISTEMS1 (DEM1) Is Essential for Cell Proliferation and Cell Differentiation in Tomato

Louisa Matthew, Melquiades E. C. Reyes, Christopher W. G. Mann, Alasdair W. McDowall, Andrew L. Eamens and Bernard J. Carroll

Plants, Vol.11(19), pp.1-27

2022

DOI: https://doi.org/10.3390/plants11192545

PMCID: PMC9573268

PMID: 36235411

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Abstract

DEFECTIVE EMBRYO AND MERISTEMS1 (DEM1) gene

cell differentiation

cell proliferation

leaf development; tomato (Solanum lycopersicum)

vegetative development

Most flowering plant species contain at least two copies of the DEFECTIVE EMBRYO AND MERISTEMS (DEM) gene with the encoded DEM proteins lacking homology to proteins of known biochemical function. In tomato (Si; Solanum lycopersicum), stable mutations in the S1DEM1 locus result in shoot and root meristem defects with the dem1 mutant failing to progress past the cotyledon stage of seedling development. Generation of a Somatic Mutagenesis of DEM1 (SMD) transformant line in tomato allowed for the characterization of S1DEM1 gene function past the seedling stage of vegetative development with SMD plants displaying a range of leaf development abnormalities. Further, the sectored or stable in planta expression of specific regions of the S1DEM1 coding sequence also resulted in the generation of tomato transformants that displayed a range of vegetative development defects, which when considered together with the dem1 mutant seedling and SMD transformant line phenotypic data, allowed for the assignment of S1DEM1 gene function to early embryo development, adaxial epidermis cell development, lateral leaf blade expansion, and mesophyll cell proliferation and differentiation.

Details

Title: DEFECTIVE EMBRYO AND MERISTEMS1 (DEM1) Is Essential for Cell Proliferation and Cell Differentiation in Tomato
Authors: Louisa Matthew (Author) - The University of Queensland
Melquiades E. C. Reyes (Author) - The University of Queensland
Christopher W. G. Mann (Author) - The University of Queensland
Alasdair W. McDowall (Author) - The University of Queensland
Andrew L. Eamens (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Health and Behavioural Sciences - Legacy
Bernard J. Carroll (Corresponding Author) - The University of Queensland
Publication details: Plants, Vol.11(19), pp.1-27
Publisher: MDPI AG
Date published: 2022
DOI: 10.3390/plants11192545
ISSN: 2223-7747
PMID: 36235411; PMC9573268
Copyright note: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Grant note: This work was supported by Australian Research Council grant CE034821 and internally by The University of Queensland to B.J.C.; L.M. and M.E.C.R. were supported by The University of Queensland PhD Scholarships, and A.L.E. was supported by a University of Queensland funded RSP2 Senior Research Fellowship.
Organisation Unit: School of Health - Biomedicine; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99681396702621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Plant Sciences

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