Cytogenetic studies of Haplopappus gracilis in both callus and suspension cell cultures

S E Ashmore; Alison Shapcott

doi:10.1007/BF00288807

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Cytogenetic studies of Haplopappus gracilis in both callus and suspension cell cultures

Journal article

Peer reviewed

Cytogenetic studies of Haplopappus gracilis in both callus and suspension cell cultures

S E Ashmore and Alison Shapcott

Theoretical and Applied Genetics: international journal of plant breeding research, Vol.78(2), pp.249-259

1989

DOI: https://doi.org/10.1007/BF00288807

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Abstract

Biological Sciences

Agricultural and Veterinary Sciences

Technology

Haplopappus gracilis

C-banding

karyotype instability

Callus culture

suspension culture

Investigations have been carried out on karyotype change in both callus and suspension cell cultures of Haplopappus gracilis (2n=4). It has been found that polyploidization arises directly in culture to give up to six times the normal diploid chromosome number in some cultures. In polyploid cultures, both chromosome loss and chromosome rearrangements occur to give rise to aneuploid karyotypes displaying chromosomes which differ in morphology from the diploid set. Whole or partial chromosome loss has been observed in the form of lagging chromosomes and chromosome bridges at anaphase, and micronuclei, ring chromosomes and chromosome fragments at other stages in mitosis. C-banded preparations have confirmed the occurrence of chromosomal rearrangements. Comparative investigations suggest that (i) more polyploidy occurs in callus cultures than in suspension cell cultures, and (ii) the presence of cytokinin (kinetin) in the culture medium may reduce the extent of karyotype change.

Details

Title: Cytogenetic studies of Haplopappus gracilis in both callus and suspension cell cultures
Authors: S E Ashmore (Author) - Griffith University
Alison Shapcott (Author) - University of Tasmania
Publication details: Theoretical and Applied Genetics: international journal of plant breeding research, Vol.78(2), pp.249-259
Publisher: Springer
Date published: 1989
DOI: 10.1007/BF00288807
ISSN: 0040-5752
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449074102621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Agronomy; Genetics & Heredity; Horticulture; Plant Sciences

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