Journal article
Culture-independent genome sequencing of clinical samples reveals an unexpected heterogeneity of infections by Chlamydia pecorum
Journal of Clinical Microbiology, Vol.53(5), pp.1573-1581
2015
Abstract
Chlamydia pecorum is an important global pathogen of livestock and it is also a significant threat to the long-term survival of Australia's koala populations. This study employed a culture-independent DNA capture approach to sequence C. pecorum genomes directly from clinical swabs samples collected from koalas with chlamydial disease as well as from sheep with arthritis and conjunctivitis. Investigations into single nucleotide polymorphisms within each of the swab samples revealed that a portion of the reads in each sample belonged to separate C. pecorum strains, suggesting that all of the clinical samples analyzed contained mixed populations of genetically distinct C. pecorum isolates. This observation was independent of the anatomical site sampled and the host species. Using the genomes of strains identified in each of these samples, whole genome phylogenetic analysis revealed that a clade containing a bovine and a koala isolate is distinct from other clades comprised of livestock or koala C. pecorum strains. Providing additional evidence to support exposure of koalas to Australian livestock strains, two 'minor' strains assembled from the koala swab samples clustered with livestock strains rather than koala strains. Culture-independent probe-based genome capture and sequencing of clinical samples provides the strongest evidence yet to suggest that naturally occurring chlamydial infections are comprised of multiple genetically distinct strains.
Details
- Title
- Culture-independent genome sequencing of clinical samples reveals an unexpected heterogeneity of infections by Chlamydia pecorum
- Authors
- Nathan Bachmann (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMitchell J Sullivan (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMartina Jelocnik (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringGarry S A Myers (Author) - University of Technology SydneyPeter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAdam Polkinghorne (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Journal of Clinical Microbiology, Vol.53(5), pp.1573-1581
- Publisher
- American Society for Microbiology
- Date published
- 2015
- DOI
- 10.1128/JCM.03534-14
- ISSN
- 0095-1137; 0095-1137
- Copyright note
- Copyright © 2015 American Society for Microbiology. Reproduced here in accordance with the publishers copyright policy.
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449332602621
- Output Type
- Journal article
- Research Statement
- false
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