Journal article
Culture-independent detection of chlorhexidine resistance genes qacA/B and smr in bacterial DNA recovered from body sites treated with chlorhexidine-containing dressings
Journal of Medical Microbiology, Vol.66(4), pp.447-453
2017
Abstract
Purpose. Dressings containing chlorhexidine gluconate (CHG) are increasingly used in clinical environments for prevention of infection at central venous catheter insertion sites. Increased tolerance to this biocide in staphylococci is primarily associated with the presence of qacA/B and smr genes. Methodology. We used a culture-independent method to assess the prevalence of these genes in 78 DNA specimens recovered from the skin of 43 patients at catheter insertion sites in the arm that were covered with CHG dressings. Results. Of the 78 DNA specimens analysed, 52 (67 %) possessed qac A/B and 14 (18 %) possessed smr; all samples positive for smr were also positive for qacA/B. These prevalence rates were not statistically greater than those observed in a subsample of specimens taken from non-CHG treated contralateral arms and non-CHG-dressing exposed arms. A statistically greater proportion of specimens with greater than 72 h exposure to CHG dressings were qac-positive (P=0.04), suggesting that the patients were contaminated with bacteria or DNA containing qacA/B during their hospital stay. The presence of qac genes was not positively associated with the presence of DNA specific for Staphylococcus epidermidis and Staphylococcus aureus in these specimens. Conclusion. Our results show that CHG genes are highly prevalent on hospital patients' skin, even in the absence of viable bacteria.
Details
- Title
- Culture-independent detection of chlorhexidine resistance genes qacA/B and smr in bacterial DNA recovered from body sites treated with chlorhexidine-containing dressings
- Authors
- Md Abu Choudhury (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringHanna E Sidjabat (Author) - University of QueenslandIrani U Rathnayake (Author) - Queensland University of TechnologyNicole Gavin (Author) - Queensland University of TechnologyRaymond J Chan (Author) - Griffith UniversityNicole Marsh (Author) - Griffith UniversityShahera Banu (Author) - Queensland University of TechnologyFlavia Huygens (Author) - Queensland University of TechnologyDavid L Paterson (Author) - Griffith UniversityClaire M Rickard (Author) - University of QueenslandDavid J McMillan (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Journal of Medical Microbiology, Vol.66(4), pp.447-453
- Publisher
- Microbiology Society
- Date published
- 2017
- DOI
- 10.1099/jmm.0.000463
- ISSN
- 0022-2615
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451197602621
- Output Type
- Journal article
Metrics
3 File views/ downloads
681 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Web Of Science research areas
- Microbiology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites