Culture-independent approaches to chlamydial genomics

Alyce Taylor-Brown; Danielle Madden; Adam Polkinghorne

doi:10.1099/mgen.0.000145

Back

Culture-independent approaches to chlamydial genomics

Journal article

Open access

Peer reviewed

Culture-independent approaches to chlamydial genomics

Alyce Taylor-Brown, Danielle Madden and Adam Polkinghorne

Microbial genomics, Vol.4

2018

DOI: https://doi.org/10.1099/mgen.0.000145

Files and links (2)

pdf

PDF - Published Version (Open Access)2.70 MBDownload View

Published VersionPDF - Published Version (Open Access)CC BY-NC V4.0, Open Access

url

https://doi.org/10.1099/mgen.0.000145View

Published Version

Abstract

culture-independent

novel species

diversity

metagenomics

chlamydiae

The expanding field of bacterial genomics has revolutionized our understanding of microbial diversity, biology and phylogeny. For most species, DNA extracted from culture material is used as the template for genome sequencing; however, the majority of microbes are actually uncultivable, and others, such as obligate intracellular bacteria, require laborious tissue culture to yield sufficient genomic material for sequencing. Chlamydiae are one such group of obligate intracellular microbes whose characterization has been hampered by this requirement. To circumvent these challenges, researchers have developed culture-independent sample preparation methods that can be applied to the sample directly or to genomic material extracted from the sample. These methods, which encompass both targeted [immunomagnetic separation-multiple displacement amplification (IMS-MDA) and sequence capture] and non-targeted approaches (host methylated DNA depletion-microbial DNA enrichment and cell-sorting-MDA), have been applied to a range of clinical and environmental samples to generate whole genomes of novel chlamydial species and strains. This review aims to provide an overview of the application, advantages and limitations of these targeted and non-targeted approaches in the chlamydial context. The methods discussed also have broad application to other obligate intracellular bacteria or clinical and environmental samples.

Details

Title: Culture-independent approaches to chlamydial genomics
Authors: Alyce Taylor-Brown (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Danielle Madden (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Adam Polkinghorne (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Microbial genomics, Vol.4; 10
Publisher: Microbiology Society
Date published: 2018
DOI: 10.1099/mgen.0.000145
ISSN: 2057-5858
Copyright note: This is an open access article published by the Microbiology Society under the Creative Commons Attribution-NonCommercial License
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99450432502621
Output Type: Journal article

Metrics

24 File views/ downloads

534 Record Views

21 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Web Of Science research areas: Genetics & Heredity; Microbiology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites