Culture-based identification to examine spatiotemporal patterns of fungal communities colonizing wood in ground contact

Paola Torres-Andrade; Jeffrey J Morrell; Jed Cappellazzi; Jeffrey K Stone

doi:10.1080/00275514.2019.1631050

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Culture-based identification to examine spatiotemporal patterns of fungal communities colonizing wood in ground contact

Journal article

Peer reviewed

Culture-based identification to examine spatiotemporal patterns of fungal communities colonizing wood in ground contact

Paola Torres-Andrade, Jeffrey J Morrell, Jed Cappellazzi and Jeffrey K Stone

Mycologia, Vol.111(5), pp.703-718

2019

DOI: https://doi.org/10.1080/00275514.2019.1631050

PMID: 31348726

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Abstract

DNA

decay

Douglas-fir

fungi

molecular identification

western redcedar

wood

red alder

soil contact

Timber durability is often assessed using small wood stakes exposed in direct soil contact, and the assessment generally emphasizes effects on wood rather than organisms involved. Understanding fungal colonization patterns can help identify key decay agents under varying conditions and use these patterns to improve wood protection strategies. Fungal colonization of red alder (Alnus rubra), Douglas-fir (Pseudotsuga menziesii) heartwood/sapwood, and western redcedar (Thuja plicata) field stakes was assessed over 2 y in western Oregon. Spatiotemporal fungal community variations were identified via culturing and DNA sequencing, where 814 isolates were identified from 84 stakes. Forty-six ascomycete genera were identified, with Phialophora, Trichoderma, and Epicoccum species occurring most frequently. Twenty-three basidiomycete genera were identified, with Trametes and Phanerochaete being the most common. Douglas-fir and western redcedar stakes contained the highest and lowest diversity levels, respectively, reflecting natural durability differences of these species. Fungal species abundance was higher below ground than in the above ground and groundline zones, likely reflecting more stable moisture regimes, proximity to soil-based fungi, and potential nutrient migration into wood beneath the soil surface. Ascomycetes were proportionally more abundant early in the exposure period, but basidiomycetes were also observed early in the process, and there appeared to be no consistent colonization pattern.

Details

Title: Culture-based identification to examine spatiotemporal patterns of fungal communities colonizing wood in ground contact
Authors: Paola Torres-Andrade (Author) - Oregon State University, United States
Jeffrey J Morrell (Author) - University of the Sunshine Coast
Jed Cappellazzi (Author) - Oregon State University, United States
Jeffrey K Stone (Author) - Oregon State University, United States
Publication details: Mycologia, Vol.111(5), pp.703-718
Publisher: Taylor & Francis Inc.
Date published: 2019
DOI: 10.1080/00275514.2019.1631050
ISSN: 0027-5514
PMID: 31348726
Organisation Unit: University of the Sunshine Coast, Queensland; National Centre for Timber Durability and Design Life; Forest Research Institute
Language: English
Record Identifier: 99451419902621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Mycology