Cryopreservation of black-lip pearl oyster (Pinctada margaritifera, L.) spermatozoa: Effects of cryoprotectants on spermatozoa motility

L Lyons; D R Jerry; Paul C Southgate

doi:10.2983/0730-8000(2005)24[1187:COBPOP]2.0.CO;2

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Cryopreservation of black-lip pearl oyster (Pinctada margaritifera, L.) spermatozoa: Effects of cryoprotectants on spermatozoa motility

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Cryopreservation of black-lip pearl oyster (Pinctada margaritifera, L.) spermatozoa: Effects of cryoprotectants on spermatozoa motility

L Lyons, D R Jerry and Paul C Southgate

Journal of Shellfish Research, Vol.24(4), pp.1187-1190

2005

DOI: https://doi.org/10.2983/0730-8000(2005)24[1187:COBPOP]2.0.CO;2

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Abstract

pearl oyster

Pinctada margaritifera

cryopreservation

spermatozoa

motility

progressive motility

CASA

Cryopreservation of sperm is seen as an important step in developing effective hatchery culture techniques for the black-lip pearl oyster, Pinctada margaritifera. As a preliminary investigation into Cryopreservation of the gametes of this species we tested 5 cryoprotectant agent combinations for their ability to retain sperm motility: (1) 1 M trehalose and 5, 10 and 15% dimethyl sulphoxide (DMSO); (2) Hanks calcium-free balanced salt solution (C-F HBSS) and 5%, 10% and 15% DMSO; (3) C-F HBSS and 5, 10 and 15% propylene glycol (PG); (4) 1 M trehalose and an equal combination of DMSO and PG making up 5, 10, 15% total volume; and (5) C-F HBSS and an equal combination of DMSO and PG making up 5%, 10% and 15% total volume. Total, rapid and progressive sperm motilities were estimated through computer assisted sperm analysis (CASA). Sperm cryopreserved in 1 M trehalose and 5% DMSO retained the highest total (86.0±1.2% SE), progressive (46.0 ±1.2% SE) and rapid (25.1±0.6% SE) motilities of all cryoprotectant solutions, whereas those cryopreserved with PG generally retained poor motility. Although the 1 M trehalose and 5% DMSO treatment compared favorably with that of fresh sperm for total motility (P < 0.01), all cryoprotectant treatments were poor at retaining the proportion of original rapid and progressively moving sperm. This study highlights the potential for cryopreservation of gametes from P. margaritifera, which will benefit selective breeding and conservation programs with this important commercial species.

Details

Title: Cryopreservation of black-lip pearl oyster (Pinctada margaritifera, L.) spermatozoa: Effects of cryoprotectants on spermatozoa motility
Authors: L Lyons (Author) - James Cook University
D R Jerry (Author) - James Cook University
Paul C Southgate (Author) - James Cook University
Publication details: Journal of Shellfish Research, Vol.24(4), pp.1187-1190
Publisher: National Shellfisheries Association, Inc.
Date published: 2005
DOI: 10.2983/0730-8000(2005)24[1187:COBPOP]2.0.CO;2
ISSN: 0730-8000
Organisation Unit: School of Science and Engineering - Legacy; Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99450101502621
Output Type: Journal article

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