Crustacean larval factor shares structural characteristics with the insect-specific follicle cell protein

Tomer Ventura; Chan D H Nguyen; Quinn P Fitzgibbon; Tomer Abramov; Gregory G Smith; Abigail Elizur

doi:10.1038/s41598-019-39173-7

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Crustacean larval factor shares structural characteristics with the insect-specific follicle cell protein

Journal article

Open access

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Crustacean larval factor shares structural characteristics with the insect-specific follicle cell protein

Tomer Ventura, Chan D H Nguyen, Quinn P Fitzgibbon, Tomer Abramov, Gregory G Smith and Abigail Elizur

Scientific Reports, Vol.9, 2847

2019

DOI: https://doi.org/10.1038/s41598-019-39173-7

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Abstract

Literature on the cuticle formation in larval stages of the diverse group of decapod crustaceans is lacking, as opposed to a wealth of knowledge in several insect groups. Here we provide the first glimpse of the cuticular organisation in larvae of the eastern spiny lobster Sagmariasus verreauxi. A bioinformatic approach applied to S. verreauxi transcriptome through metamorphosis identified for the first time a small secreted protein with multiple isoforms that is highly expressed in crustacean larvae. This protein, named crustacean larval factor (Clf) shares structural characteristics with insect follicle cell protein 3 (FCP3), an insect-specific, rapidly evolving protein, with spatial-temporal regulated expression that is restricted to follicular cells during the production of the vitellin coat. Furthermore, we identified the FCP3 domain in additional structural proteins in multiple arthropod groups. Recombinant Clf inhibited in vitro calcium carbonate crystalline precipitation, in keeping with the finding that the spiny lobster larval cuticle is mainly composed of amorphous calcium carbonate. In addition, the recombinant Clf was shown to bind chitosan. Taken together, this research identifies two novel structural domains with lineage-specific expansion across arthropods. In crustaceans, Clf is found predominantly in larvae and the spatial-temporal regulated FCP3 factor occurs as a domain identified in multiple structural proteins across arthropods. Given the shared ten cysteines backbone between the Clf and FCP domains, a shared evolution is suggested and should be further explored.

Details

Title: Crustacean larval factor shares structural characteristics with the insect-specific follicle cell protein
Authors: Tomer Ventura (Author) - University of the Sunshine Coast - School of Science & Engineering
Chan D H Nguyen (Author) - University of the Sunshine Coast - School of Science & Engineering
Quinn P Fitzgibbon (Author) - University of Tasmania
Tomer Abramov (Author) - University of the Sunshine Coast - School of Science & Engineering
Gregory G Smith (Author) - University of Tasmania
Abigail Elizur (Author) - University of the Sunshine Coast - School of Science & Engineering
Publication details: Scientific Reports, Vol.9, 2847
Publisher: Nature Publishing Group
Date published: 2019
DOI: 10.1038/s41598-019-39173-7
ISSN: 2045-2322
Copyright note: Copyright © 2019 the Authors. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Grants: Redefining the molecular mechanism underlying crustacean metamorphosis, DP160103320, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Science and Engineering - Legacy; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450721102621
Output Type: Journal article

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