Cost-effective interrogation of single nucleotide polymorphisms using the mismatch amplification mutation assay and capillary electrophoresis

Erin P Price; M A Matthews; J A Beaudry; J L Allred; J M Schupp; D N Birdsell; T Pearson; P Keim

doi:10.1002/elps.201000379

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Cost-effective interrogation of single nucleotide polymorphisms using the mismatch amplification mutation assay and capillary electrophoresis

Journal article

Open access

Peer reviewed

Cost-effective interrogation of single nucleotide polymorphisms using the mismatch amplification mutation assay and capillary electrophoresis

Erin P Price, M A Matthews, J A Beaudry, J L Allred, J M Schupp, D N Birdsell, T Pearson and P Keim

Electrophoresis, Vol.31(23-24), pp.3881-3888

2010

DOI: https://doi.org/10.1002/elps.201000379

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Abstract

bacillus anthracis

CE universal tail mismatch amplification mutation assay

genotyping

SNP

The ability to characterize SNPs is an important aspect of many clinical diagnostic, genetic and evolutionary studies. Here, we designed a multiplexed SNP genotyping method to survey a large number of phylogenetically informative SNPs within the genome of the bacterium Bacillus anthracis. This novel method, CE universal tail mismatch amplification mutation assay (CUMA), allows for PCR multiplexing and automatic scoring of SNP genotypes, thus providing a rapid, economical and higher throughput alternative to more expensive SNP genotyping techniques. CUMA delivered accurate B. anthracis SNP genotyping results and, when multiplexed, saved reagent costs by more than 80% compared with TaqMan real-time PCR. When real-time PCR technology and instrumentation is unavailable or the reagents are cost-prohibitive, CUMA is a powerful alternative for SNP genotyping. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Details

Title: Cost-effective interrogation of single nucleotide polymorphisms using the mismatch amplification mutation assay and capillary electrophoresis
Authors: Erin P Price (Author) - Northern Arizona University, United States
M A Matthews (Author) - Northern Arizona University, United States
J A Beaudry (Author) - Northern Arizona University, United States
J L Allred (Author) - Northern Arizona University, United States
J M Schupp (Author) - Northern Arizona University, United States
D N Birdsell (Author) - Northern Arizona University, United States
T Pearson (Author) - Northern Arizona University, United States
P Keim (Author) - Northern Arizona University, United States
Publication details: Electrophoresis, Vol.31(23-24), pp.3881-3888
Publisher: Wiley - V C H Verlag GmbH & Co. KGaA
Date published: 2010
DOI: 10.1002/elps.201000379
ISSN: 0173-0835
Copyright note: Copyright © 2010 American Society for Microbiology. The author's accepted version is reproduced here in accordance with the publisher's copyright policy. The definitive version is available at http://aem.asm.org/
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99451039402621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemical Research Methods; Chemistry, Analytical

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