Journal article
Coronary artery bypass grafting is associated with immunoparalysis of monocytes and dendritic cells
Journal of Cellular and Molecular Medicine, Vol.24(8), pp.4791-4803
2020
Abstract
Coronary artery bypass grafting (CABG) triggers a systemic inflammatory response that may contribute to adverse outcomes. Dendritic cells (DC) and monocytes are immunoregulatory cells potentially affected by CABG, contributing to an altered immune state. This study investigated changes in DC and monocyte responses in CABG patients at 5 time-points: admission, peri-operative, ICU, day 3 and day 5. Whole blood from 49 CABG patients was used in an ex vivo whole blood culture model to prospectively assess DC and monocyte responses. Lipopolysaccharide (LPS) was added in parallel to model responses to an infectious complication. Co-stimulatory and adhesion molecule expression and intracellular mediator production was measured by flow cytometry. CABG modulated monocyte and DC responses. In addition, DC and monocytes were immunoparalysed, evidenced by failure of co-stimulatory and adhesion molecules (eg HLA-DR), and intracellular mediators (eg IL-6) to respond to LPS stimulation. DC and monocyte modulation was associated with prolonged ICU length of stay and post-operative atrial fibrillation. DC and monocyte cytokine production did not recover by day 5 post-surgery. This study provides evidence that CABG modulates DC and monocyte responses. Using an ex vivo model to assess immune competency of CABG patients may help identify biomarkers to predict adverse outcomes.
Details
- Title
- Coronary artery bypass grafting is associated with immunoparalysis of monocytes and dendritic cells
- Authors
- Alexis J Perros (Author) - 61USC_INST___lifebloodArlanna Esguerra-Lallen (Author) - Australian Red Cross LifebloodKelly Rooks (Author) - Australian Red Cross LifebloodFenny Chong (Author) - Australian Red Cross LifebloodSanne Engkilde-Pederssen (Author) - Australian Red Cross LifebloodHelen M Faddy (Author) - University of the Sunshine Coast - School of Health and Sport SciencesElise Hewlett (Author) - Australian Red Cross LifebloodRishendran Naidoo (Author) - Prince Charles HospitalJohn-Paul Tung (Author) - The University of QueenslandJohn F Fraser (Author) - The University of QueenslandPeter Tesar (Author) - Prince Charles HospitalMarc Ziegenfuss (Author) - Prince Charles HospitalSusan Smith (Author) - Prince Charles HospitalDonalee O'Brien (Author) - Prince Charles HospitalRobert L Flower (Author) - Queensland University of TechnologyMelinda M Dean (Author) - University of the Sunshine Coast - School of Health and Sport Sciences
- Publication details
- Journal of Cellular and Molecular Medicine, Vol.24(8), pp.4791-4803
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2020
- DOI
- 10.1111/jcmm.15154
- ISSN
- 1582-1838
- Copyright note
- Copyright © 2020 Australian Red Cross Lifeblood. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99450843102621
- Output Type
- Journal article
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