Core-Shell Beads as Microreactors for Phylogrouping of E. coli Strains

Lena Gorgannezhad; Kamalalayam Rajan Sreejith; Melody Christie; Jing Jin; Chin Hong Ooi; Mohammad Katouli; Helen Stratton; Nam-Trung Nguyen

doi:10.3390/mi11080761

Back

Core-Shell Beads as Microreactors for Phylogrouping of E. coli Strains

Journal article

Open access

Peer reviewed

Core-Shell Beads as Microreactors for Phylogrouping of E. coli Strains

Lena Gorgannezhad, Kamalalayam Rajan Sreejith, Melody Christie, Jing Jin, Chin Hong Ooi, Mohammad Katouli, Helen Stratton and Nam-Trung Nguyen

Micromachines , Vol.11(8), pp.1-11

2020

DOI: https://doi.org/10.3390/mi11080761

PMCID: PMC7464145

PMID: 32784703

Files and links (2)

pdf

Core-Shell Beads as Microreactors for Phylogrouping of E coli Strains1.15 MBDownload View

Published Version Open Access CC BY V4.0

url

https://doi.org/10.3390/mi11080761View

Published Version Open

Abstract

PCR

liquid marble

core-shell bead

phylogrouping

simultaneous detection

Multiplex polymerase chain reaction (PCR) is an effective tool for simultaneous detection of target genes. Nevertheless, their use has been restricted due to the intrinsic interference between primer pairs. Performing several single PCRs in an array format instead of a multiplex PCR is a simple way to overcome this obstacle. However, there are still major technical challenges in designing a new generation of single PCR microreactors with a small sample volume, rapid thermal cycling, and no evaporation during amplification. We report a simple and robust core-shell bead array for a series of single amplifications. Four core-shell beads with a polymer coating and PCR mixture were synthesized using liquid marble formation and subsequent photo polymerization. Each bead can detect one target gene. We constructed a customised system for thermal cycling of these core-shell beads. Phylogrouping of the E. coli strains was carried out based on the fluorescent signal of the core-shell beads. This platform can be a promising alternative for multiplex nucleic acid analyses due to its simplicity and high throughput. The platform reported here also reduces the cycling time and avoids evaporation as well as contamination of the sample during the amplification process.

Details

Title: Core-Shell Beads as Microreactors for Phylogrouping of E. coli Strains
Authors: Lena Gorgannezhad (Author) - Griffith University
Kamalalayam Rajan Sreejith (Author) - Griffith University
Melody Christie (Author) - Griffith University
Jing Jin (Author) - Griffith University
Chin Hong Ooi (Author) - Griffith University
Mohammad Katouli (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Helen Stratton (Author) - Griffith University
Nam-Trung Nguyen (Author) - Griffith University
Publication details: Micromachines , Vol.11(8), pp.1-11
Publisher: MDPI AG
Date published: 2020
DOI: 10.3390/mi11080761
ISSN: 2072-666X
PMID: 32784703; PMC7464145
Copyright note: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Grants: Engineering floating liquid marbles for three-dimensional cell cultures, DP170100277, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99482302802621
Output Type: Journal article

Metrics

31 File views/ downloads

47 Record Views

10 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Web Of Science research areas: Chemistry, Analytical; Instruments & Instrumentation; Nanoscience & Nanotechnology; Physics, Applied

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: SDGs from InCites