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Published VersionCC BY V4.0, Open Access
Journal article
Core-Shell Beads as Microreactors for Phylogrouping of E. coli Strains
Micromachines , Vol.11(8), pp.1-11
2020
Abstract
Multiplex polymerase chain reaction (PCR) is an effective tool for simultaneous detection of target genes. Nevertheless, their use has been restricted due to the intrinsic interference between primer pairs. Performing several single PCRs in an array format instead of a multiplex PCR is a simple way to overcome this obstacle. However, there are still major technical challenges in designing a new generation of single PCR microreactors with a small sample volume, rapid thermal cycling, and no evaporation during amplification. We report a simple and robust core-shell bead array for a series of single amplifications. Four core-shell beads with a polymer coating and PCR mixture were synthesized using liquid marble formation and subsequent photo polymerization. Each bead can detect one target gene. We constructed a customised system for thermal cycling of these core-shell beads. Phylogrouping of the E. coli strains was carried out based on the fluorescent signal of the core-shell beads. This platform can be a promising alternative for multiplex nucleic acid analyses due to its simplicity and high throughput. The platform reported here also reduces the cycling time and avoids evaporation as well as contamination of the sample during the amplification process.
Details
- Title
- Core-Shell Beads as Microreactors for Phylogrouping of E. coli Strains
- Authors
- Lena Gorgannezhad (Author) - Griffith UniversityKamalalayam Rajan Sreejith (Author) - Griffith UniversityMelody Christie (Author) - Griffith UniversityJing Jin (Author) - Griffith UniversityChin Hong Ooi (Author) - Griffith UniversityMohammad Katouli (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyHelen Stratton (Author) - Griffith UniversityNam-Trung Nguyen (Author) - Griffith University
- Publication details
- Micromachines , Vol.11(8), pp.1-11
- Publisher
- MDPI AG
- Date published
- 2020
- DOI
- 10.3390/mi11080761
- ISSN
- 2072-666X
- Copyright note
- © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Organisation Unit
- School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99482302802621
- Output Type
- Journal article
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- Chemistry, Analytical
- Instruments & Instrumentation
- Nanoscience & Nanotechnology
- Physics, Applied
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